Electricity production and microbial biofilm characterization in cellulose-fed microbial fuel cells

Z. Ren, L. M. Steinberg, J. M. Regan

Research output: Contribution to journalArticle

84 Scopus citations

Abstract

Converting biodegradable materials into electricity, microbial fuel cells (MFCs) present a promising technology for renewable energy production in specific applications. Unlike typical soluble substrates that have been used as electron donors in MFC studies, cellulose is unique because it requires a microbial consortium that can metabolize both an insoluble electron donor (cellulose) and electron acceptor (electrode). In this study, electricity generation and the microbial ecology of cellulose-fed MFCs were analyzed using a defined co-culture of Clostridium cellulolyticum and Geobacter sulfurreducens. Fluorescent in situ hybridization and quantitative PCR showed that when particulate MN301 cellulose was used as sole substrate, most Clostridium cells were found adhered to cellulose particles in suspension, while most Geobacter cells were attached to the electrode. By comparison, both bacteria resided in suspension and biofilm samples when soluble carboxymethyl cellulose was used. This distinct function-related distribution of the bacteria suggests an opportunity to optimize reactor operation by settling cellulose and decanting supernatant to extend cellulose hydrolysis and improve cellulose-electricity conversion.

Original languageEnglish (US)
Pages (from-to)617-622
Number of pages6
JournalWater Science and Technology
Volume58
Issue number3
DOIs
StatePublished - Oct 6 2008

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Water Science and Technology

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