Electrochemical Measurements Reveal Reactive Oxygen Species in Stress Granules**

Keke Hu, Emily Relton, Nicolas Locker, Nhu T.N. Phan, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

Stress granules (SGs) are membrane-less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite-induced SGs, and H2O2 is the main species. The release kinetics of H2O2 from single SGs and the number of H2O2 molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections.

Original languageEnglish (US)
Pages (from-to)15302-15306
Number of pages5
JournalAngewandte Chemie - International Edition
Volume60
Issue number28
DOIs
StatePublished - Jul 5 2021

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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