Electrogenic reduction of the primary electron donor P700+ in photosystem I by redox dyes

Kira N. Gourovskaya, Mahir D. Mamedov, Ilya R. Vassiliev, John H. Golbeck, Alexey Yu Semenov

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Abstract

The kinetics of reduction of the photo-oxidized primary electron donor P700+ by redox dyes N,N,N',N'tetremethyl-p-phenylendiamine, 2,6-dichlorophenol-indophenol and phenazine methosulfate was studied in proteoliposomes containing Photosystem I complexes from cyanobacteria Synechocystis sp. PCC 6803 using direct electrometrical technique, In the presence of high concentrations of redox dyes, the fast generation of a membrane potential related to electron transfer between P700 and the terminal iron-sulfur clusters F(A)/F(B) was followed by a new electrogenic phase in the millisecond time domain, which contributes approximately 20% to the overall photoelectric response. Tills phase is ascribed to the vectorial transfer of an electron from the redox dye to the protein-embedded chlorophyll of P700+. Since the contribution of this electrogenic phase in the presence of artificial redox dyes is approximately equal to that of the phase observed earlier in the presence of cytochrome c6, it is likely that electrogenic reduction of P700+ in vivo occurs due to vectorial electron transfer within RC molecule rather than within the cytochrome c6-P700 complex.

Original languageEnglish (US)
Pages (from-to)193-196
Number of pages4
JournalFEBS Letters
Volume414
Issue number2
DOIs
Publication statusPublished - Sep 8 1997

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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