Electrogenicity accompanies photoreduction of the iron-sulfur clusters F(A) and F(B) in photosystem I

Mahir D. Mamedov, Kira N. Gourovskaya, Ilya R. Vassiliev, John H. Golbeck, Alexey Yu Sememov

Research output: Contribution to journalArticle

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Abstract

Photovoltage responses accompanying electron transfer on the acceptor side of photosystem I (PS I) were investigated in proteoliposomes containing PS I complexes from the cyanobacterium Synechococcus sp. PCC 6301 using a direct electrochemical technique. The relative contributions of the F(X) → F(B) and the F(X) → F(A) electron transfer reactions to the overall electrogenicity were elucidated by comparing the sodium dithionite-induced decrease in the magnitude of the total photoelectric responses in control and in F(B)-less (HgCl2-treated) PS I complexes. The results obtained suggest that the electrogenesis on the acceptor side of PS I is related to electron transfers between both F(X) and F(A) and F(A) and F(B). Based on the electrogenic nature of the latter reaction in PS I complexes, we conclude that F(A) rather than F(B) is the acceptor proximal to F(X).

Original languageEnglish (US)
Pages (from-to)219-223
Number of pages5
JournalFEBS Letters
Volume431
Issue number2
DOIs
StatePublished - Jul 17 1998

Fingerprint

Photosystem I Protein Complex
Sulfur
Iron
Electrons
Electrochemical Techniques
Synechococcus
Dithionite
Mercuric Chloride
Cyanobacteria

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Mamedov, Mahir D. ; Gourovskaya, Kira N. ; Vassiliev, Ilya R. ; Golbeck, John H. ; Sememov, Alexey Yu. / Electrogenicity accompanies photoreduction of the iron-sulfur clusters F(A) and F(B) in photosystem I. In: FEBS Letters. 1998 ; Vol. 431, No. 2. pp. 219-223.
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abstract = "Photovoltage responses accompanying electron transfer on the acceptor side of photosystem I (PS I) were investigated in proteoliposomes containing PS I complexes from the cyanobacterium Synechococcus sp. PCC 6301 using a direct electrochemical technique. The relative contributions of the F(X) → F(B) and the F(X) → F(A) electron transfer reactions to the overall electrogenicity were elucidated by comparing the sodium dithionite-induced decrease in the magnitude of the total photoelectric responses in control and in F(B)-less (HgCl2-treated) PS I complexes. The results obtained suggest that the electrogenesis on the acceptor side of PS I is related to electron transfers between both F(X) and F(A) and F(A) and F(B). Based on the electrogenic nature of the latter reaction in PS I complexes, we conclude that F(A) rather than F(B) is the acceptor proximal to F(X).",
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Electrogenicity accompanies photoreduction of the iron-sulfur clusters F(A) and F(B) in photosystem I. / Mamedov, Mahir D.; Gourovskaya, Kira N.; Vassiliev, Ilya R.; Golbeck, John H.; Sememov, Alexey Yu.

In: FEBS Letters, Vol. 431, No. 2, 17.07.1998, p. 219-223.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrogenicity accompanies photoreduction of the iron-sulfur clusters F(A) and F(B) in photosystem I

AU - Mamedov, Mahir D.

AU - Gourovskaya, Kira N.

AU - Vassiliev, Ilya R.

AU - Golbeck, John H.

AU - Sememov, Alexey Yu

PY - 1998/7/17

Y1 - 1998/7/17

N2 - Photovoltage responses accompanying electron transfer on the acceptor side of photosystem I (PS I) were investigated in proteoliposomes containing PS I complexes from the cyanobacterium Synechococcus sp. PCC 6301 using a direct electrochemical technique. The relative contributions of the F(X) → F(B) and the F(X) → F(A) electron transfer reactions to the overall electrogenicity were elucidated by comparing the sodium dithionite-induced decrease in the magnitude of the total photoelectric responses in control and in F(B)-less (HgCl2-treated) PS I complexes. The results obtained suggest that the electrogenesis on the acceptor side of PS I is related to electron transfers between both F(X) and F(A) and F(A) and F(B). Based on the electrogenic nature of the latter reaction in PS I complexes, we conclude that F(A) rather than F(B) is the acceptor proximal to F(X).

AB - Photovoltage responses accompanying electron transfer on the acceptor side of photosystem I (PS I) were investigated in proteoliposomes containing PS I complexes from the cyanobacterium Synechococcus sp. PCC 6301 using a direct electrochemical technique. The relative contributions of the F(X) → F(B) and the F(X) → F(A) electron transfer reactions to the overall electrogenicity were elucidated by comparing the sodium dithionite-induced decrease in the magnitude of the total photoelectric responses in control and in F(B)-less (HgCl2-treated) PS I complexes. The results obtained suggest that the electrogenesis on the acceptor side of PS I is related to electron transfers between both F(X) and F(A) and F(A) and F(B). Based on the electrogenic nature of the latter reaction in PS I complexes, we conclude that F(A) rather than F(B) is the acceptor proximal to F(X).

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