Electrokinetic bioprocessor for concentrating cells and molecules

Pak Kin Wong, Che Yang Chen, Tza Huei Wang, Chih Ming Ho

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.

Original languageEnglish (US)
Pages (from-to)6908-6914
Number of pages7
JournalAnalytical Chemistry
Volume76
Issue number23
DOIs
StatePublished - Jan 3 2005

Fingerprint

Electroosmosis
Electrodes
Molecules
Bacteriophages
Single-Stranded DNA
Microfluidics
Escherichia coli
Flow fields
Bacteria
DNA

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Wong, Pak Kin ; Chen, Che Yang ; Wang, Tza Huei ; Ho, Chih Ming. / Electrokinetic bioprocessor for concentrating cells and molecules. In: Analytical Chemistry. 2005 ; Vol. 76, No. 23. pp. 6908-6914.
@article{e11a170cdbb249f5a0f28e42fceacb22,
title = "Electrokinetic bioprocessor for concentrating cells and molecules",
abstract = "Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.",
author = "Wong, {Pak Kin} and Chen, {Che Yang} and Wang, {Tza Huei} and Ho, {Chih Ming}",
year = "2005",
month = "1",
day = "3",
doi = "10.1021/ac049479u",
language = "English (US)",
volume = "76",
pages = "6908--6914",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "23",

}

Electrokinetic bioprocessor for concentrating cells and molecules. / Wong, Pak Kin; Chen, Che Yang; Wang, Tza Huei; Ho, Chih Ming.

In: Analytical Chemistry, Vol. 76, No. 23, 03.01.2005, p. 6908-6914.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrokinetic bioprocessor for concentrating cells and molecules

AU - Wong, Pak Kin

AU - Chen, Che Yang

AU - Wang, Tza Huei

AU - Ho, Chih Ming

PY - 2005/1/3

Y1 - 2005/1/3

N2 - Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.

AB - Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor then utilizes electrophoretic and dielectrophoretic forces, which are effective in short range, to trap the target cells and molecules on the electrode surface. By optimizing the operating parameters, we have concentrated various biological objects in a large range of sizes, including Escherichia coli bacteria, λ phage DNA, and single-stranded DNA fragments as small as 20 bases that have a radius of gyration of only 3 nm.

UR - http://www.scopus.com/inward/record.url?scp=10044278305&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10044278305&partnerID=8YFLogxK

U2 - 10.1021/ac049479u

DO - 10.1021/ac049479u

M3 - Article

VL - 76

SP - 6908

EP - 6914

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 23

ER -