Electrolyzed oxidizing water treatment for decontamination of raw salmon inoculated with Escherichia coli O157:H7 and Listeria monocytogenes Scott A and response surface modeling

Nil P. Ozer, Ali Demirci

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Raw fish is prone to the risk of microbial outbreaks due to contamination by pathogenic microorganisms, such as Escherichia coli O157:H7 and Listeria monocytogenes. Therefore, it is essential to treat raw fish to inactivate pathogenic microorganisms. Electrolyzed Oxidizing Water (EO) is a novel antimicrobial agent containing acidic solution with a pH of 2.6, Oxidation Reduction Potential (ORP) of 1150 mV, and 70-90 ppm free chlorine, and alkaline solution with a pH of 11.4 and ORP of -795 mV. This study was undertaken to evaluate the efficacy of acidic EO water treatment and alkaline EO water treatment followed by acidic EO water treatment at various temperatures for the inactivation of E. coli O157:H7 and L. monocytogenes Scott A on the muscle and skin surfaces of inoculated salmon fillets. Inoculated salmon fillets were treated with acidic EO water at 22 and 35 °C and 90 ppm free-chlorine solution as control at 22 °C for 2, 4, 8, 16, 32, and 64 min. The acidic EO water treatments resulted in a reduction of L. monocytogenes Scott A population in the range of 0.40 log10 CFU/g (60%) at 22 °C to 1.12 log 10 CFU/g (92.3%) at 35 °C. Treatment of inoculated salmon fillets with acidic EO water reduced E. coli O157:H7 populations by 0.49 log 10 CFU/g (67%) at 22 °C and 1.07 log10 CFU/g (91.1%) at 35 °C. The maximum reduction with chlorine solution (control) was 1.46 log10 CFU/g (96.3%) for E. coli O157:H7 and 1.3 log10 CFU/g (95.3%) for L. monocytogenes Scott A at 64 min. A response surface model was developed for alkaline treatment followed by acidic EO water treatment to predict treatment times in the range of 5-30 min and temperatures in the range of 22-35 °C for effective treatment with alkaline EO water followed by acidic water, alkaline and acidic water treatments. Response surface analysis demonstrated maximum log reductions of 1.33 log10 CFU/g (95.3%) for E. coli O157:H7 and 1.09 log10 CFU/g (91.9%) for L. monocytogenes Scott A. Data collected from the treatments was used to develop empirical models as a function of treatment times and temperature for prediction of population of E. coli O157:H7 and L. monocytogenes Scott A. Correlations (R2) of 0.52 and 0.77 were obtained between model predicted and experimental log 10 reduction for E. coli O157:H7 and L. monocytogenes Scott A reductions, respectively. These results clearly indicated that EO water has a potential to be used for decontamination of raw fish.

Original languageEnglish (US)
Pages (from-to)234-241
Number of pages8
JournalJournal of Food Engineering
Volume72
Issue number3
DOIs
StatePublished - Jan 1 2006

Fingerprint

Escherichia coli O157
Decontamination
Water Purification
Salmon
decontamination
Listeria monocytogenes
water treatment
salmon
Water
water
raw fish
Chlorine
fillets
chlorine
Fishes
redox potential
Temperature
Oxidation-Reduction
inactivation temperature
microorganisms

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

@article{e0b664854fd44b8ebe0b191f81bd9ff9,
title = "Electrolyzed oxidizing water treatment for decontamination of raw salmon inoculated with Escherichia coli O157:H7 and Listeria monocytogenes Scott A and response surface modeling",
abstract = "Raw fish is prone to the risk of microbial outbreaks due to contamination by pathogenic microorganisms, such as Escherichia coli O157:H7 and Listeria monocytogenes. Therefore, it is essential to treat raw fish to inactivate pathogenic microorganisms. Electrolyzed Oxidizing Water (EO) is a novel antimicrobial agent containing acidic solution with a pH of 2.6, Oxidation Reduction Potential (ORP) of 1150 mV, and 70-90 ppm free chlorine, and alkaline solution with a pH of 11.4 and ORP of -795 mV. This study was undertaken to evaluate the efficacy of acidic EO water treatment and alkaline EO water treatment followed by acidic EO water treatment at various temperatures for the inactivation of E. coli O157:H7 and L. monocytogenes Scott A on the muscle and skin surfaces of inoculated salmon fillets. Inoculated salmon fillets were treated with acidic EO water at 22 and 35 °C and 90 ppm free-chlorine solution as control at 22 °C for 2, 4, 8, 16, 32, and 64 min. The acidic EO water treatments resulted in a reduction of L. monocytogenes Scott A population in the range of 0.40 log10 CFU/g (60{\%}) at 22 °C to 1.12 log 10 CFU/g (92.3{\%}) at 35 °C. Treatment of inoculated salmon fillets with acidic EO water reduced E. coli O157:H7 populations by 0.49 log 10 CFU/g (67{\%}) at 22 °C and 1.07 log10 CFU/g (91.1{\%}) at 35 °C. The maximum reduction with chlorine solution (control) was 1.46 log10 CFU/g (96.3{\%}) for E. coli O157:H7 and 1.3 log10 CFU/g (95.3{\%}) for L. monocytogenes Scott A at 64 min. A response surface model was developed for alkaline treatment followed by acidic EO water treatment to predict treatment times in the range of 5-30 min and temperatures in the range of 22-35 °C for effective treatment with alkaline EO water followed by acidic water, alkaline and acidic water treatments. Response surface analysis demonstrated maximum log reductions of 1.33 log10 CFU/g (95.3{\%}) for E. coli O157:H7 and 1.09 log10 CFU/g (91.9{\%}) for L. monocytogenes Scott A. Data collected from the treatments was used to develop empirical models as a function of treatment times and temperature for prediction of population of E. coli O157:H7 and L. monocytogenes Scott A. Correlations (R2) of 0.52 and 0.77 were obtained between model predicted and experimental log 10 reduction for E. coli O157:H7 and L. monocytogenes Scott A reductions, respectively. These results clearly indicated that EO water has a potential to be used for decontamination of raw fish.",
author = "Ozer, {Nil P.} and Ali Demirci",
year = "2006",
month = "1",
day = "1",
doi = "10.1016/j.jfoodeng.2004.11.038",
language = "English (US)",
volume = "72",
pages = "234--241",
journal = "Journal of Food Engineering",
issn = "0260-8774",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Electrolyzed oxidizing water treatment for decontamination of raw salmon inoculated with Escherichia coli O157:H7 and Listeria monocytogenes Scott A and response surface modeling

AU - Ozer, Nil P.

AU - Demirci, Ali

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Raw fish is prone to the risk of microbial outbreaks due to contamination by pathogenic microorganisms, such as Escherichia coli O157:H7 and Listeria monocytogenes. Therefore, it is essential to treat raw fish to inactivate pathogenic microorganisms. Electrolyzed Oxidizing Water (EO) is a novel antimicrobial agent containing acidic solution with a pH of 2.6, Oxidation Reduction Potential (ORP) of 1150 mV, and 70-90 ppm free chlorine, and alkaline solution with a pH of 11.4 and ORP of -795 mV. This study was undertaken to evaluate the efficacy of acidic EO water treatment and alkaline EO water treatment followed by acidic EO water treatment at various temperatures for the inactivation of E. coli O157:H7 and L. monocytogenes Scott A on the muscle and skin surfaces of inoculated salmon fillets. Inoculated salmon fillets were treated with acidic EO water at 22 and 35 °C and 90 ppm free-chlorine solution as control at 22 °C for 2, 4, 8, 16, 32, and 64 min. The acidic EO water treatments resulted in a reduction of L. monocytogenes Scott A population in the range of 0.40 log10 CFU/g (60%) at 22 °C to 1.12 log 10 CFU/g (92.3%) at 35 °C. Treatment of inoculated salmon fillets with acidic EO water reduced E. coli O157:H7 populations by 0.49 log 10 CFU/g (67%) at 22 °C and 1.07 log10 CFU/g (91.1%) at 35 °C. The maximum reduction with chlorine solution (control) was 1.46 log10 CFU/g (96.3%) for E. coli O157:H7 and 1.3 log10 CFU/g (95.3%) for L. monocytogenes Scott A at 64 min. A response surface model was developed for alkaline treatment followed by acidic EO water treatment to predict treatment times in the range of 5-30 min and temperatures in the range of 22-35 °C for effective treatment with alkaline EO water followed by acidic water, alkaline and acidic water treatments. Response surface analysis demonstrated maximum log reductions of 1.33 log10 CFU/g (95.3%) for E. coli O157:H7 and 1.09 log10 CFU/g (91.9%) for L. monocytogenes Scott A. Data collected from the treatments was used to develop empirical models as a function of treatment times and temperature for prediction of population of E. coli O157:H7 and L. monocytogenes Scott A. Correlations (R2) of 0.52 and 0.77 were obtained between model predicted and experimental log 10 reduction for E. coli O157:H7 and L. monocytogenes Scott A reductions, respectively. These results clearly indicated that EO water has a potential to be used for decontamination of raw fish.

AB - Raw fish is prone to the risk of microbial outbreaks due to contamination by pathogenic microorganisms, such as Escherichia coli O157:H7 and Listeria monocytogenes. Therefore, it is essential to treat raw fish to inactivate pathogenic microorganisms. Electrolyzed Oxidizing Water (EO) is a novel antimicrobial agent containing acidic solution with a pH of 2.6, Oxidation Reduction Potential (ORP) of 1150 mV, and 70-90 ppm free chlorine, and alkaline solution with a pH of 11.4 and ORP of -795 mV. This study was undertaken to evaluate the efficacy of acidic EO water treatment and alkaline EO water treatment followed by acidic EO water treatment at various temperatures for the inactivation of E. coli O157:H7 and L. monocytogenes Scott A on the muscle and skin surfaces of inoculated salmon fillets. Inoculated salmon fillets were treated with acidic EO water at 22 and 35 °C and 90 ppm free-chlorine solution as control at 22 °C for 2, 4, 8, 16, 32, and 64 min. The acidic EO water treatments resulted in a reduction of L. monocytogenes Scott A population in the range of 0.40 log10 CFU/g (60%) at 22 °C to 1.12 log 10 CFU/g (92.3%) at 35 °C. Treatment of inoculated salmon fillets with acidic EO water reduced E. coli O157:H7 populations by 0.49 log 10 CFU/g (67%) at 22 °C and 1.07 log10 CFU/g (91.1%) at 35 °C. The maximum reduction with chlorine solution (control) was 1.46 log10 CFU/g (96.3%) for E. coli O157:H7 and 1.3 log10 CFU/g (95.3%) for L. monocytogenes Scott A at 64 min. A response surface model was developed for alkaline treatment followed by acidic EO water treatment to predict treatment times in the range of 5-30 min and temperatures in the range of 22-35 °C for effective treatment with alkaline EO water followed by acidic water, alkaline and acidic water treatments. Response surface analysis demonstrated maximum log reductions of 1.33 log10 CFU/g (95.3%) for E. coli O157:H7 and 1.09 log10 CFU/g (91.9%) for L. monocytogenes Scott A. Data collected from the treatments was used to develop empirical models as a function of treatment times and temperature for prediction of population of E. coli O157:H7 and L. monocytogenes Scott A. Correlations (R2) of 0.52 and 0.77 were obtained between model predicted and experimental log 10 reduction for E. coli O157:H7 and L. monocytogenes Scott A reductions, respectively. These results clearly indicated that EO water has a potential to be used for decontamination of raw fish.

UR - http://www.scopus.com/inward/record.url?scp=24144471225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24144471225&partnerID=8YFLogxK

U2 - 10.1016/j.jfoodeng.2004.11.038

DO - 10.1016/j.jfoodeng.2004.11.038

M3 - Article

VL - 72

SP - 234

EP - 241

JO - Journal of Food Engineering

JF - Journal of Food Engineering

SN - 0260-8774

IS - 3

ER -