Electron cryotomography

C. M. Oikonomou, M. T. Swulius, A. Briegel, M. Beeby, Q. Yao, G. J. Jensen

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Electron cryotomography (ECT) delivers 3D images of native structures inside intact cells with resolution on the scale of large macromolecular complexes (~ 4 nm). It has proven to be an invaluable tool for interrogating the organization of bacterial cells and the structures of the nanomachines inside them. Here we present a brief introduction to the technique, a few examples of what we have learned by imaging bacterial cells with ECT over the last 15 years and a frank discussion of the relative advantages and limitations of the technique compared to some other popular imaging methods used in microbiology.

Original languageEnglish (US)
Title of host publicationImaging Bacterial Molecules, Structures and Cells, 2016
EditorsGrant J. Jensen, Colin Harwood
PublisherAcademic Press Inc.
Pages115-139
Number of pages25
ISBN (Print)9780128093924
DOIs
Publication statusPublished - Jan 1 2016

Publication series

NameMethods in Microbiology
Volume43
ISSN (Print)0580-9517

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Oikonomou, C. M., Swulius, M. T., Briegel, A., Beeby, M., Yao, Q., & Jensen, G. J. (2016). Electron cryotomography. In G. J. Jensen, & C. Harwood (Eds.), Imaging Bacterial Molecules, Structures and Cells, 2016 (pp. 115-139). (Methods in Microbiology; Vol. 43). Academic Press Inc.. https://doi.org/10.1016/bs.mim.2016.10.001