Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution

Hyeran Noh, Naris Barnthip, Purnendu Parhi, Erwin A. Vogler

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.

Original languageEnglish (US)
Title of host publicationNanomaterial Interfaces in Biology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages157-166
Number of pages10
ISBN (Print)9781627034616
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1025
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Noh, H., Barnthip, N., Parhi, P., & Vogler, E. A. (2013). Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. In Nanomaterial Interfaces in Biology: Methods and Protocols (pp. 157-166). (Methods in Molecular Biology; Vol. 1025). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-462-3_12