Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution

Hyeran Noh, Naris Barnthip, Purnendu Parhi, Erwin A. Vogler

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.

Original languageEnglish (US)
Title of host publicationNanomaterial Interfaces in Biology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages157-166
Number of pages10
ISBN (Print)9781627034616
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1025
ISSN (Print)1064-3745

Fingerprint

Adsorption
Proteins
Sepharose
Glass
Electrophoresis
Gels

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Noh, H., Barnthip, N., Parhi, P., & Vogler, E. A. (2013). Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. In Nanomaterial Interfaces in Biology: Methods and Protocols (pp. 157-166). (Methods in Molecular Biology; Vol. 1025). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-462-3_12
Noh, Hyeran ; Barnthip, Naris ; Parhi, Purnendu ; Vogler, Erwin A. / Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. Nanomaterial Interfaces in Biology: Methods and Protocols. Humana Press Inc., 2013. pp. 157-166 (Methods in Molecular Biology).
@inbook{a371728b1ab4499ba74848388aa13f78,
title = "Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution",
abstract = "The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.",
author = "Hyeran Noh and Naris Barnthip and Purnendu Parhi and Vogler, {Erwin A.}",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/978-1-62703-462-3_12",
language = "English (US)",
isbn = "9781627034616",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "157--166",
booktitle = "Nanomaterial Interfaces in Biology",

}

Noh, H, Barnthip, N, Parhi, P & Vogler, EA 2013, Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. in Nanomaterial Interfaces in Biology: Methods and Protocols. Methods in Molecular Biology, vol. 1025, Humana Press Inc., pp. 157-166. https://doi.org/10.1007/978-1-62703-462-3_12

Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. / Noh, Hyeran; Barnthip, Naris; Parhi, Purnendu; Vogler, Erwin A.

Nanomaterial Interfaces in Biology: Methods and Protocols. Humana Press Inc., 2013. p. 157-166 (Methods in Molecular Biology; Vol. 1025).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution

AU - Noh, Hyeran

AU - Barnthip, Naris

AU - Parhi, Purnendu

AU - Vogler, Erwin A.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.

AB - The venerable solution-depletion method is perhaps the most unambiguous method of measuring solute adsorption from solution to solid particles, requiring neither complex instrumentation nor associated interpretive theory. We describe herein an SDS-gel electrophoresis implementation of the solution-depletion method for measuring protein adsorption and protein-adsorption kinetics. Silanized-glass particles with different surface chemistry/energy and hydrophobic sepharose-based chromatographic media are used as example adsorbents. Electrophoretic separation enables quantification of adsorption competition among multiple proteins in solution for the same adsorbent surface, demonstrated herein by adsorption-competition kinetics from binary solution.

UR - http://www.scopus.com/inward/record.url?scp=84934444897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934444897&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-462-3_12

DO - 10.1007/978-1-62703-462-3_12

M3 - Chapter

SN - 9781627034616

T3 - Methods in Molecular Biology

SP - 157

EP - 166

BT - Nanomaterial Interfaces in Biology

PB - Humana Press Inc.

ER -

Noh H, Barnthip N, Parhi P, Vogler EA. Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution. In Nanomaterial Interfaces in Biology: Methods and Protocols. Humana Press Inc. 2013. p. 157-166. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-462-3_12