Electrophysiologic effects of intracellular lysophosphoglycerides and their accumulation in cardiac lymph with myocardial ischemia in dogs

H. Akita, Michael Creer, K. A. Yamada, B. E. Sobel, P. B. Corr

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lysophosphatidylcholine (LPC) accumulates in ischemic tissue, and exogenous LPC (20-100 μM) induces electrophysiologic alterations in vitro. However, to determine whether compartmentalization is critical, intracellular pressure microinjection of LPC was performed with simultaneous recording of the transmembrane action potential. Intracellular LPC in concentrations as high as 500 μM (n = 18), calculated based on calibration of injectate volume and cellular volume, did not induce electrophysiologic alterations. The concentrations and efflux of phospholipids and lysophospholipids were assessed in lymph obtained from the supracardiac lymph vessel in anesthetized dogs to assess the extent of extracellular accumulation. Prior to ischemia, phosphatidylcholine (PC) was the major phospholipid in lymph (79±2%) with substantial quantities of sphingomyelin (11±2%) and LPC (6±1%). With ischemia, the concentration of LPC increased by 18%, and net efflux of LPC increased by 24% (P < 0.01) with no net efflux of PC or other assayed phospholipids. The calculated concentration of LPC increased from 84 to 197 μM in lymph within the ischemic region, a concentration sufficient to induce electrophysiologic derangements.

Original languageEnglish (US)
Pages (from-to)271-280
Number of pages10
JournalJournal of Clinical Investigation
Volume78
Issue number1
DOIs
StatePublished - Jan 1 1986

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Lysophosphatidylcholines
Lymph
Myocardial Ischemia
Dogs
Phospholipids
Phosphatidylcholines
Ischemia
Lysophospholipids
Sphingomyelins
lysophosphoglycerides
Microinjections
Membrane Potentials
Calibration
Action Potentials
Pressure

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Electrophysiologic effects of intracellular lysophosphoglycerides and their accumulation in cardiac lymph with myocardial ischemia in dogs",
abstract = "Lysophosphatidylcholine (LPC) accumulates in ischemic tissue, and exogenous LPC (20-100 μM) induces electrophysiologic alterations in vitro. However, to determine whether compartmentalization is critical, intracellular pressure microinjection of LPC was performed with simultaneous recording of the transmembrane action potential. Intracellular LPC in concentrations as high as 500 μM (n = 18), calculated based on calibration of injectate volume and cellular volume, did not induce electrophysiologic alterations. The concentrations and efflux of phospholipids and lysophospholipids were assessed in lymph obtained from the supracardiac lymph vessel in anesthetized dogs to assess the extent of extracellular accumulation. Prior to ischemia, phosphatidylcholine (PC) was the major phospholipid in lymph (79±2{\%}) with substantial quantities of sphingomyelin (11±2{\%}) and LPC (6±1{\%}). With ischemia, the concentration of LPC increased by 18{\%}, and net efflux of LPC increased by 24{\%} (P < 0.01) with no net efflux of PC or other assayed phospholipids. The calculated concentration of LPC increased from 84 to 197 μM in lymph within the ischemic region, a concentration sufficient to induce electrophysiologic derangements.",
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Electrophysiologic effects of intracellular lysophosphoglycerides and their accumulation in cardiac lymph with myocardial ischemia in dogs. / Akita, H.; Creer, Michael; Yamada, K. A.; Sobel, B. E.; Corr, P. B.

In: Journal of Clinical Investigation, Vol. 78, No. 1, 01.01.1986, p. 271-280.

Research output: Contribution to journalArticle

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