Electrophysiological effects of cholinergic agonists in surface epithelium of Necturus gastric antrum

Audrey E. Gadacz, Mary E. Klingensmith, David Soybel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Intracellular microelectrode techniques were used to characterize voltage and conductance properties of the basolateral membrane of surface epithelial cells in in vitro Necturus antral mucosa. Flux studies confirmed that this tissue secretes HCO 3 / - under resting conditions and during response to cholinergic stimulation. In studies using intracellular microelectrodes, exposure to cholinergic agonists such as acetylcholine, bethanechol, or carbachol elicited an initial hyperpolarization followed by depolarization of the basolateral cell membrane associated with up to fourfold increases in basolateral membrane conductance. Effects of acetylcholine were dose dependent (10 -6 -10 -4 M) and prevented by pretreatment of tissues with the nonselective muscarinic receptor blocker atropine. Some variation in this response to cholinergic stimulation was observed and appeared to be related to the season (fall/winter/early spring vs. late spring/summer). Despite such variability, circuit analysis and ion-substitution studies indicated that the carbachol-induced increases in basolateral conductance were due to increases in conductance to K + and Cl - . These increases in basolateral transport processes may serve to stabilize cell ion composition and membrane electrical properties during cholinergic stimulation of mucus and HCO 3 / - secretions.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume270
Issue number3 33-3
StatePublished - Apr 15 1996

Fingerprint

Necturus
Pyloric Antrum
Cholinergic Agonists
Cholinergic Agents
Epithelium
Carbachol
Microelectrodes
Acetylcholine
Membranes
Ions
Bethanechol
Muscarinic Receptors
Mucus
Atropine
Mucous Membrane
Epithelial Cells
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

@article{e11d9f5d625d464f83cd62b55c64e9d2,
title = "Electrophysiological effects of cholinergic agonists in surface epithelium of Necturus gastric antrum",
abstract = "Intracellular microelectrode techniques were used to characterize voltage and conductance properties of the basolateral membrane of surface epithelial cells in in vitro Necturus antral mucosa. Flux studies confirmed that this tissue secretes HCO 3 / - under resting conditions and during response to cholinergic stimulation. In studies using intracellular microelectrodes, exposure to cholinergic agonists such as acetylcholine, bethanechol, or carbachol elicited an initial hyperpolarization followed by depolarization of the basolateral cell membrane associated with up to fourfold increases in basolateral membrane conductance. Effects of acetylcholine were dose dependent (10 -6 -10 -4 M) and prevented by pretreatment of tissues with the nonselective muscarinic receptor blocker atropine. Some variation in this response to cholinergic stimulation was observed and appeared to be related to the season (fall/winter/early spring vs. late spring/summer). Despite such variability, circuit analysis and ion-substitution studies indicated that the carbachol-induced increases in basolateral conductance were due to increases in conductance to K + and Cl - . These increases in basolateral transport processes may serve to stabilize cell ion composition and membrane electrical properties during cholinergic stimulation of mucus and HCO 3 / - secretions.",
author = "Gadacz, {Audrey E.} and Klingensmith, {Mary E.} and David Soybel",
year = "1996",
month = "4",
day = "15",
language = "English (US)",
volume = "270",
journal = "American Journal of Physiology",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "3 33-3",

}

Electrophysiological effects of cholinergic agonists in surface epithelium of Necturus gastric antrum. / Gadacz, Audrey E.; Klingensmith, Mary E.; Soybel, David.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 270, No. 3 33-3, 15.04.1996.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrophysiological effects of cholinergic agonists in surface epithelium of Necturus gastric antrum

AU - Gadacz, Audrey E.

AU - Klingensmith, Mary E.

AU - Soybel, David

PY - 1996/4/15

Y1 - 1996/4/15

N2 - Intracellular microelectrode techniques were used to characterize voltage and conductance properties of the basolateral membrane of surface epithelial cells in in vitro Necturus antral mucosa. Flux studies confirmed that this tissue secretes HCO 3 / - under resting conditions and during response to cholinergic stimulation. In studies using intracellular microelectrodes, exposure to cholinergic agonists such as acetylcholine, bethanechol, or carbachol elicited an initial hyperpolarization followed by depolarization of the basolateral cell membrane associated with up to fourfold increases in basolateral membrane conductance. Effects of acetylcholine were dose dependent (10 -6 -10 -4 M) and prevented by pretreatment of tissues with the nonselective muscarinic receptor blocker atropine. Some variation in this response to cholinergic stimulation was observed and appeared to be related to the season (fall/winter/early spring vs. late spring/summer). Despite such variability, circuit analysis and ion-substitution studies indicated that the carbachol-induced increases in basolateral conductance were due to increases in conductance to K + and Cl - . These increases in basolateral transport processes may serve to stabilize cell ion composition and membrane electrical properties during cholinergic stimulation of mucus and HCO 3 / - secretions.

AB - Intracellular microelectrode techniques were used to characterize voltage and conductance properties of the basolateral membrane of surface epithelial cells in in vitro Necturus antral mucosa. Flux studies confirmed that this tissue secretes HCO 3 / - under resting conditions and during response to cholinergic stimulation. In studies using intracellular microelectrodes, exposure to cholinergic agonists such as acetylcholine, bethanechol, or carbachol elicited an initial hyperpolarization followed by depolarization of the basolateral cell membrane associated with up to fourfold increases in basolateral membrane conductance. Effects of acetylcholine were dose dependent (10 -6 -10 -4 M) and prevented by pretreatment of tissues with the nonselective muscarinic receptor blocker atropine. Some variation in this response to cholinergic stimulation was observed and appeared to be related to the season (fall/winter/early spring vs. late spring/summer). Despite such variability, circuit analysis and ion-substitution studies indicated that the carbachol-induced increases in basolateral conductance were due to increases in conductance to K + and Cl - . These increases in basolateral transport processes may serve to stabilize cell ion composition and membrane electrical properties during cholinergic stimulation of mucus and HCO 3 / - secretions.

UR - http://www.scopus.com/inward/record.url?scp=0029958860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029958860&partnerID=8YFLogxK

M3 - Article

VL - 270

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0193-1849

IS - 3 33-3

ER -