Electroporation in the developing mouse olfactory bulb

Fumiaki Imamura, Charles A. Greer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In utero electroporation is a method to deliver DNA into the developing mouse brain. The technique is very useful to study the mechanisms of mammalian brain development, and now has been widely applied to many brain regions and species since the method was first reported by Saito and Nakatsuji in 2001. Here, we provide a protocol for applying in utero electroporation to the developing mouse olfactory bulb (OB). The target cells of this protocol in the OB are the projection neurons, especially mitral cells. By introducing different plasmids, our method allows us to follow the developmental changes of mitral cell location and morphology in the presumptive OB of the embryonic mouse brain, and to study functions of specific molecules in developing mitral cells. Mitral cells are generated during a narrow temporal window in the embryonic brain, and unfortunately, there are few effective gene-targeted or transgenic mouse lines for studying the development of OB projection neurons. The electroporation technique we describe here can overcome these disadvantages, and therefore, will facilitate the study of the molecular mechanisms regulating mitral cell development. In utero electroporation that can be used to track mitral cell migration and differentiation as well as to upregulate or downregulate the candidate developmental molecules will lead us to a deeper understanding of organization within the olfactory system and the strategies it employs for processing odor information.

Original languageEnglish (US)
Title of host publicationElectroporation Methods in Neuroscience
PublisherSpringer New York
Pages69-79
Number of pages11
ISBN (Electronic)9781493924592
ISBN (Print)9781493924585
DOIs
StatePublished - Mar 9 2015

Fingerprint

Electroporation
Olfactory Bulb
Brain
Neurons
Automatic Data Processing
Transgenic Mice
Cell Movement
Cell Differentiation
Plasmids
Up-Regulation
Down-Regulation
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Neuroscience(all)

Cite this

Imamura, F., & Greer, C. A. (2015). Electroporation in the developing mouse olfactory bulb. In Electroporation Methods in Neuroscience (pp. 69-79). Springer New York. https://doi.org/10.1007/978-1-4939-2459-2_5
Imamura, Fumiaki ; Greer, Charles A. / Electroporation in the developing mouse olfactory bulb. Electroporation Methods in Neuroscience. Springer New York, 2015. pp. 69-79
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Imamura, F & Greer, CA 2015, Electroporation in the developing mouse olfactory bulb. in Electroporation Methods in Neuroscience. Springer New York, pp. 69-79. https://doi.org/10.1007/978-1-4939-2459-2_5

Electroporation in the developing mouse olfactory bulb. / Imamura, Fumiaki; Greer, Charles A.

Electroporation Methods in Neuroscience. Springer New York, 2015. p. 69-79.

Research output: Chapter in Book/Report/Conference proceedingChapter

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Imamura F, Greer CA. Electroporation in the developing mouse olfactory bulb. In Electroporation Methods in Neuroscience. Springer New York. 2015. p. 69-79 https://doi.org/10.1007/978-1-4939-2459-2_5