Electrospray analysis of proteins: A comparison of positive‐ion and negative‐ion mass spectra at high and low pH

Michele A. Kelly, Martha M. Vestling, Catherine C. Fenselau, Philip B. Smith

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

Cationic and anionic electrospray mass spectra were measured for equine myoglobin, lactalbumin and hen egg white lysozyme from acidic and basic solutions. Both positive and negative ions were detected from all solutions in which the proteins were soluble. This was observed in both nebulization‐assisted and thermally assisted electrospray. As in other studies, the distribution of the charge states reflected functional groups in the proteins, and also folding or extension of the protein.

Original languageEnglish (US)
Pages (from-to)1143-1147
Number of pages5
JournalOrganic Mass Spectrometry
Volume27
Issue number10
DOIs
StatePublished - Oct 1992

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Instrumentation

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