Electrospun bio-nanocomposite scaffolds for bone tissue engineering by cellulose nanocrystals reinforcing maleic anhydride grafted PLA

Chengjun Zhou, Qingfeng Shi, Weihong Guo, Lekeith Terrell, Ammar T. Qureshi, Daniel J. Hayes, Qinglin Wu

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Electrospun fibrous bio-nanocomposite scaffolds reinforced with cellulose nanocrystals (CNCs) were fabricated by using maleic anhydride (MAH) grafted poly(lactic acid) (PLA) as matrix with improved interfacial adhesion between the two components. Morphological, thermal, mechanical, and in vitro degradation properties as well as basic cytocompatibility using human adult adipose derived mesenchymal stem cells (hASCs) of MAH grafted PLA/CNC (i.e., MPLA/CNC) scaffolds were characterized. Morphological investigation indicated that the diameter and polydispersity of electrospun MPLA/CNC nanofibers were reduced with the increased CNC content. The addition of CNCs improved both the thermal stability and mechanical properties of MPLA/CNC composites. The MPLA/CNC scaffolds at the 5 wt % CNC loading level showed not only superior tensile strength (more than 10 MPa), but also improved stability during in vitro degradation compared with the MPLA and PLA/CNC counterparts. Moreover, the fibrous MPLA/CNC composite scaffolds were non-toxic to hASCs and capable of supporting cell proliferation. This study demonstrates that fibrous MPLA/CNC bio-nanocomposite scaffolds are biodegradable, cytocompatible, and possess useful mechanical properties for bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)3847-3854
Number of pages8
JournalACS Applied Materials and Interfaces
Volume5
Issue number9
DOIs
StatePublished - May 8 2013

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Maleic Anhydrides
Maleic anhydride
Lactic acid
Scaffolds (biology)
Tissue engineering
Cellulose
Nanocrystals
Nanocomposites
Bone
Scaffolds
Stem cells
poly(lactic acid)
Degradation
Mechanical properties
Composite materials
Cell proliferation
Polydispersity
Nanofibers

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Zhou, Chengjun ; Shi, Qingfeng ; Guo, Weihong ; Terrell, Lekeith ; Qureshi, Ammar T. ; Hayes, Daniel J. ; Wu, Qinglin. / Electrospun bio-nanocomposite scaffolds for bone tissue engineering by cellulose nanocrystals reinforcing maleic anhydride grafted PLA. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 9. pp. 3847-3854.
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abstract = "Electrospun fibrous bio-nanocomposite scaffolds reinforced with cellulose nanocrystals (CNCs) were fabricated by using maleic anhydride (MAH) grafted poly(lactic acid) (PLA) as matrix with improved interfacial adhesion between the two components. Morphological, thermal, mechanical, and in vitro degradation properties as well as basic cytocompatibility using human adult adipose derived mesenchymal stem cells (hASCs) of MAH grafted PLA/CNC (i.e., MPLA/CNC) scaffolds were characterized. Morphological investigation indicated that the diameter and polydispersity of electrospun MPLA/CNC nanofibers were reduced with the increased CNC content. The addition of CNCs improved both the thermal stability and mechanical properties of MPLA/CNC composites. The MPLA/CNC scaffolds at the 5 wt {\%} CNC loading level showed not only superior tensile strength (more than 10 MPa), but also improved stability during in vitro degradation compared with the MPLA and PLA/CNC counterparts. Moreover, the fibrous MPLA/CNC composite scaffolds were non-toxic to hASCs and capable of supporting cell proliferation. This study demonstrates that fibrous MPLA/CNC bio-nanocomposite scaffolds are biodegradable, cytocompatible, and possess useful mechanical properties for bone tissue engineering.",
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Electrospun bio-nanocomposite scaffolds for bone tissue engineering by cellulose nanocrystals reinforcing maleic anhydride grafted PLA. / Zhou, Chengjun; Shi, Qingfeng; Guo, Weihong; Terrell, Lekeith; Qureshi, Ammar T.; Hayes, Daniel J.; Wu, Qinglin.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 9, 08.05.2013, p. 3847-3854.

Research output: Contribution to journalArticle

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