Electrospun PGS: PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy

Nafiseh Masoumi, Benjamin L. Larson, Nasim Annabi, Mahshid Kharaziha, Behnam Zamanian, Kayle S. Shapero, Alexander T. Cubberley, Gulden Camci-Unal, Keefe B. Manning, John E. Mayer, Ali Khademhosseini

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Tissue engineered heart valves (TEHV) can be useful in the repair of congenital or acquired valvular diseases due to their potential for growth and remodeling. The development of biomimetic scaffolds is a major challenge in heart valve tissue engineering. One of the most important structural characteristics of mature heart valve leaflets is their intrinsic anisotropy, which is derived from the microstructure of aligned collagen fibers in the extracellular matrix (ECM). In the present study, a directional electrospinning technique is used to fabricate fibrous poly(glycerol sebacate):poly(caprolactone) (PGS:PCL) scaffolds containing aligned fibers, which resemble native heart valve leaflet ECM networks. In addition, the anisotropic mechanical characteristics of fabricated scaffolds are tuned by changing the ratio of PGS:PCL to mimic the native heart valve's mechanical properties. Primary human valvular interstitial cells (VICs) attach and align along the anisotropic axes of all PGS:PCL scaffolds with various mechanical properties. The cells are also biochemically active in producing heart-valve-associated collagen, vimentin, and smooth muscle actin as determined by gene expression. The fibrous PGS:PCL scaffolds seeded with human VICs mimick the structure and mechanical properties of native valve leaflet tissues and would potentially be suitable for the replacement of heart valves in diverse patient populations. A novel scaffold containing fiber structures is fabricated resembling the ECM networks in the native heart valve leaflet. Directional electrospinning is used to create tunable anisotropic composite materials with varying ratios of PGS:PCL. The human valvular interstitial cells (VICs) are found to be organized and aligned along the anisotropic axes of the composite TEHVs.

Original languageEnglish (US)
Pages (from-to)929-939
Number of pages11
JournalAdvanced Healthcare Materials
Volume3
Issue number6
DOIs
StatePublished - Jun 2014

Fingerprint

Heart Valves
Anisotropy
Scaffolds
Glycerol
Extracellular Matrix
Electrospinning
Collagen
Mechanical properties
Fibers
Tissue
Biomimetics
Composite materials
Vimentin
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Gene expression
Smooth Muscle
Muscle
Actins

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Masoumi, N., Larson, B. L., Annabi, N., Kharaziha, M., Zamanian, B., Shapero, K. S., ... Khademhosseini, A. (2014). Electrospun PGS: PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy. Advanced Healthcare Materials, 3(6), 929-939. https://doi.org/10.1002/adhm.201300505
Masoumi, Nafiseh ; Larson, Benjamin L. ; Annabi, Nasim ; Kharaziha, Mahshid ; Zamanian, Behnam ; Shapero, Kayle S. ; Cubberley, Alexander T. ; Camci-Unal, Gulden ; Manning, Keefe B. ; Mayer, John E. ; Khademhosseini, Ali. / Electrospun PGS : PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy. In: Advanced Healthcare Materials. 2014 ; Vol. 3, No. 6. pp. 929-939.
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abstract = "Tissue engineered heart valves (TEHV) can be useful in the repair of congenital or acquired valvular diseases due to their potential for growth and remodeling. The development of biomimetic scaffolds is a major challenge in heart valve tissue engineering. One of the most important structural characteristics of mature heart valve leaflets is their intrinsic anisotropy, which is derived from the microstructure of aligned collagen fibers in the extracellular matrix (ECM). In the present study, a directional electrospinning technique is used to fabricate fibrous poly(glycerol sebacate):poly(caprolactone) (PGS:PCL) scaffolds containing aligned fibers, which resemble native heart valve leaflet ECM networks. In addition, the anisotropic mechanical characteristics of fabricated scaffolds are tuned by changing the ratio of PGS:PCL to mimic the native heart valve's mechanical properties. Primary human valvular interstitial cells (VICs) attach and align along the anisotropic axes of all PGS:PCL scaffolds with various mechanical properties. The cells are also biochemically active in producing heart-valve-associated collagen, vimentin, and smooth muscle actin as determined by gene expression. The fibrous PGS:PCL scaffolds seeded with human VICs mimick the structure and mechanical properties of native valve leaflet tissues and would potentially be suitable for the replacement of heart valves in diverse patient populations. A novel scaffold containing fiber structures is fabricated resembling the ECM networks in the native heart valve leaflet. Directional electrospinning is used to create tunable anisotropic composite materials with varying ratios of PGS:PCL. The human valvular interstitial cells (VICs) are found to be organized and aligned along the anisotropic axes of the composite TEHVs.",
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Masoumi, N, Larson, BL, Annabi, N, Kharaziha, M, Zamanian, B, Shapero, KS, Cubberley, AT, Camci-Unal, G, Manning, KB, Mayer, JE & Khademhosseini, A 2014, 'Electrospun PGS: PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy', Advanced Healthcare Materials, vol. 3, no. 6, pp. 929-939. https://doi.org/10.1002/adhm.201300505

Electrospun PGS : PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy. / Masoumi, Nafiseh; Larson, Benjamin L.; Annabi, Nasim; Kharaziha, Mahshid; Zamanian, Behnam; Shapero, Kayle S.; Cubberley, Alexander T.; Camci-Unal, Gulden; Manning, Keefe B.; Mayer, John E.; Khademhosseini, Ali.

In: Advanced Healthcare Materials, Vol. 3, No. 6, 06.2014, p. 929-939.

Research output: Contribution to journalArticle

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T1 - Electrospun PGS

T2 - PCL Microfibers Align Human Valvular Interstitial Cells and Provide Tunable Scaffold Anisotropy

AU - Masoumi, Nafiseh

AU - Larson, Benjamin L.

AU - Annabi, Nasim

AU - Kharaziha, Mahshid

AU - Zamanian, Behnam

AU - Shapero, Kayle S.

AU - Cubberley, Alexander T.

AU - Camci-Unal, Gulden

AU - Manning, Keefe B.

AU - Mayer, John E.

AU - Khademhosseini, Ali

PY - 2014/6

Y1 - 2014/6

N2 - Tissue engineered heart valves (TEHV) can be useful in the repair of congenital or acquired valvular diseases due to their potential for growth and remodeling. The development of biomimetic scaffolds is a major challenge in heart valve tissue engineering. One of the most important structural characteristics of mature heart valve leaflets is their intrinsic anisotropy, which is derived from the microstructure of aligned collagen fibers in the extracellular matrix (ECM). In the present study, a directional electrospinning technique is used to fabricate fibrous poly(glycerol sebacate):poly(caprolactone) (PGS:PCL) scaffolds containing aligned fibers, which resemble native heart valve leaflet ECM networks. In addition, the anisotropic mechanical characteristics of fabricated scaffolds are tuned by changing the ratio of PGS:PCL to mimic the native heart valve's mechanical properties. Primary human valvular interstitial cells (VICs) attach and align along the anisotropic axes of all PGS:PCL scaffolds with various mechanical properties. The cells are also biochemically active in producing heart-valve-associated collagen, vimentin, and smooth muscle actin as determined by gene expression. The fibrous PGS:PCL scaffolds seeded with human VICs mimick the structure and mechanical properties of native valve leaflet tissues and would potentially be suitable for the replacement of heart valves in diverse patient populations. A novel scaffold containing fiber structures is fabricated resembling the ECM networks in the native heart valve leaflet. Directional electrospinning is used to create tunable anisotropic composite materials with varying ratios of PGS:PCL. The human valvular interstitial cells (VICs) are found to be organized and aligned along the anisotropic axes of the composite TEHVs.

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