Electrostatic effects on protein partitioning in size-exclusion chromatography and membrane ultrafiltration

Narahari S. Pujar, Andrew L. Zydney

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Although size-exclusion chromatography and membrane ultrafiltration are generally viewed as size-based separation processes, there is considerable evidence for the importance of electrostatic interactions. Experimental studies of sizeexclusion chromatography and membrane ultrafiltration were performed in parallel using both neutral dextrans and charged proteins. Data for protein retention time and membrane sieving clearly indicate that the effective protein size increases with decreasing ionic strength clue to the reduction in electrostatic shielding. These results were quantified using available theoretical models for the partitioning of charged solutes. The data clearly demonstrate the similarity of the electrostatic interactions and partitioning effects in size-exclusion chromatography and membrane ultrafiltration.

Original languageEnglish (US)
Pages (from-to)229-238
Number of pages10
JournalJournal of Chromatography A
Volume796
Issue number2
DOIs
StatePublished - Feb 20 1998

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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