Elevated interstitial adenosine concentrations do not activate the muscle reflex

David A. Maclean, Lisa M. Vickery, Lawrence Sinoway

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effects of adenosine perfusion of the isolated triceps surae muscle group in the decerebrate cat on interstitial adenosine concentrations as well as heart rate and blood pressure responses. In six male cats (6.0 ± 0.21 kg), the triceps surae muscle group of both legs was perfused with an artificial blood solution containing no additives (control) and then with blood containing 20 mM or 100 μM adenosine for 10 min. An intact muscle reflex was confirmed by bolus injections of 50 mM phosphate and/or saturated KCl administered into the triceps surae muscle via the cannulated popliteal artery before and after adenosine blood perfusion. Microdialysis of the triceps surae muscle group during muscle perfusion revealed that interstitial adenosine was elevated (P < 0.05) from 0.9 ± 0.3 μM during control blood perfusion to 2,421 ± 547 μM during 20 mM adenosine perfusion. In addition, interstitial adenosine levels were increased (P < 0.05) from 1.1 ± 0.3 μM during control blood perfusion to 4.1 ± 1.2 μM during perfusion with 100 μM adenosine. Despite the large increases in interstitial adenosine levels, perfusion of the triceps surae muscle group with the two blood adenosine solutions resulted in no significant increases in heart rate or blood pressure. These data strongly suggest that elevated interstitial adenosine concentrations do not play a role in activating the muscle reflex and confirm our previous in vivo human findings.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume280
Issue number2 49-2
StatePublished - Feb 1 2001

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Adenosine
Reflex
Muscles
Perfusion
Cats
Heart Rate
Blood Pressure
Blood Substitutes
Popliteal Artery
Microdialysis
Leg
Phosphates
Injections

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

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Elevated interstitial adenosine concentrations do not activate the muscle reflex. / Maclean, David A.; Vickery, Lisa M.; Sinoway, Lawrence.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 280, No. 2 49-2, 01.02.2001.

Research output: Contribution to journalArticle

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