Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis

Xiaojun Chen, Wei Li, Yalin Li, Lei Xu, Sha Zhou, Jifeng Zhu, Zhipeng Xu, Feng Liu, Dandan Lin, Fei Hu, Yuemin Liu, Wen Jiang, Liwang Cui, Chuan Su

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The pathology associated with Schistosoma japonicum (S. japonicum) infection in humans is attributed to parasite egg-induced granulomatous inflammation and fibrosis in the host liver. Currently, a marker that is reliable, cheap, less device-dependent, and can be easily and repeatedly used on a large scale to monitor the progression of liver pathology in schistosomiasis japonica endemic areas is lacking. The levels of serum S. japonicum heat shock protein 60 (SjHSP60)-specific IgG and its subtype antibodies in animals (mice and rabbits) or patients with schistosomiasis were measured by ELISA. Liver pathologies in mice and rabbits were evaluated by gross pathology and histopathology, and hepatic fibrosis in patients was examined with ultrasound imaging. The results revealed that the titers of the total IgG and subtype IgG1 anti-SjHSP60 antibodies were positively correlated with the severity of liver pathology after S. japonicum infection. Our findings indicate that the SjHSP60 IgG and IgG1 antibody levels can be used as potential candidate biomarkers for evaluation of liver pathology in schistosomiasis; however, validation remains to be explored in further work.

Original languageEnglish (US)
Article number7765
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Schistosoma japonicum
Schistosomiasis
Biomarkers
Pathology
Chaperonin 60
Immunoglobulin G
Antibodies
Liver
Serum
Fibrosis
Schistosomiasis japonica
Rabbits
Infection
Ovum
Ultrasonography
Parasites
Enzyme-Linked Immunosorbent Assay
Inflammation
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • General

Cite this

Chen, Xiaojun ; Li, Wei ; Li, Yalin ; Xu, Lei ; Zhou, Sha ; Zhu, Jifeng ; Xu, Zhipeng ; Liu, Feng ; Lin, Dandan ; Hu, Fei ; Liu, Yuemin ; Jiang, Wen ; Cui, Liwang ; Su, Chuan. / Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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title = "Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis",
abstract = "The pathology associated with Schistosoma japonicum (S. japonicum) infection in humans is attributed to parasite egg-induced granulomatous inflammation and fibrosis in the host liver. Currently, a marker that is reliable, cheap, less device-dependent, and can be easily and repeatedly used on a large scale to monitor the progression of liver pathology in schistosomiasis japonica endemic areas is lacking. The levels of serum S. japonicum heat shock protein 60 (SjHSP60)-specific IgG and its subtype antibodies in animals (mice and rabbits) or patients with schistosomiasis were measured by ELISA. Liver pathologies in mice and rabbits were evaluated by gross pathology and histopathology, and hepatic fibrosis in patients was examined with ultrasound imaging. The results revealed that the titers of the total IgG and subtype IgG1 anti-SjHSP60 antibodies were positively correlated with the severity of liver pathology after S. japonicum infection. Our findings indicate that the SjHSP60 IgG and IgG1 antibody levels can be used as potential candidate biomarkers for evaluation of liver pathology in schistosomiasis; however, validation remains to be explored in further work.",
author = "Xiaojun Chen and Wei Li and Yalin Li and Lei Xu and Sha Zhou and Jifeng Zhu and Zhipeng Xu and Feng Liu and Dandan Lin and Fei Hu and Yuemin Liu and Wen Jiang and Liwang Cui and Chuan Su",
year = "2017",
month = "12",
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Chen, X, Li, W, Li, Y, Xu, L, Zhou, S, Zhu, J, Xu, Z, Liu, F, Lin, D, Hu, F, Liu, Y, Jiang, W, Cui, L & Su, C 2017, 'Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis', Scientific reports, vol. 7, no. 1, 7765. https://doi.org/10.1038/s41598-017-08283-5

Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis. / Chen, Xiaojun; Li, Wei; Li, Yalin; Xu, Lei; Zhou, Sha; Zhu, Jifeng; Xu, Zhipeng; Liu, Feng; Lin, Dandan; Hu, Fei; Liu, Yuemin; Jiang, Wen; Cui, Liwang; Su, Chuan.

In: Scientific reports, Vol. 7, No. 1, 7765, 01.12.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated serum antibody against Schistosoma japonicum HSP60 as a promising biomarker for liver pathology in schistosomiasis

AU - Chen, Xiaojun

AU - Li, Wei

AU - Li, Yalin

AU - Xu, Lei

AU - Zhou, Sha

AU - Zhu, Jifeng

AU - Xu, Zhipeng

AU - Liu, Feng

AU - Lin, Dandan

AU - Hu, Fei

AU - Liu, Yuemin

AU - Jiang, Wen

AU - Cui, Liwang

AU - Su, Chuan

PY - 2017/12/1

Y1 - 2017/12/1

N2 - The pathology associated with Schistosoma japonicum (S. japonicum) infection in humans is attributed to parasite egg-induced granulomatous inflammation and fibrosis in the host liver. Currently, a marker that is reliable, cheap, less device-dependent, and can be easily and repeatedly used on a large scale to monitor the progression of liver pathology in schistosomiasis japonica endemic areas is lacking. The levels of serum S. japonicum heat shock protein 60 (SjHSP60)-specific IgG and its subtype antibodies in animals (mice and rabbits) or patients with schistosomiasis were measured by ELISA. Liver pathologies in mice and rabbits were evaluated by gross pathology and histopathology, and hepatic fibrosis in patients was examined with ultrasound imaging. The results revealed that the titers of the total IgG and subtype IgG1 anti-SjHSP60 antibodies were positively correlated with the severity of liver pathology after S. japonicum infection. Our findings indicate that the SjHSP60 IgG and IgG1 antibody levels can be used as potential candidate biomarkers for evaluation of liver pathology in schistosomiasis; however, validation remains to be explored in further work.

AB - The pathology associated with Schistosoma japonicum (S. japonicum) infection in humans is attributed to parasite egg-induced granulomatous inflammation and fibrosis in the host liver. Currently, a marker that is reliable, cheap, less device-dependent, and can be easily and repeatedly used on a large scale to monitor the progression of liver pathology in schistosomiasis japonica endemic areas is lacking. The levels of serum S. japonicum heat shock protein 60 (SjHSP60)-specific IgG and its subtype antibodies in animals (mice and rabbits) or patients with schistosomiasis were measured by ELISA. Liver pathologies in mice and rabbits were evaluated by gross pathology and histopathology, and hepatic fibrosis in patients was examined with ultrasound imaging. The results revealed that the titers of the total IgG and subtype IgG1 anti-SjHSP60 antibodies were positively correlated with the severity of liver pathology after S. japonicum infection. Our findings indicate that the SjHSP60 IgG and IgG1 antibody levels can be used as potential candidate biomarkers for evaluation of liver pathology in schistosomiasis; however, validation remains to be explored in further work.

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