TY - JOUR
T1 - Elioraea tepida, sp. Nov., a moderately thermophilic aerobic anoxygenic phototrophic bacterium isolated from the mat community of an alkaline siliceous hot spring in yellowstone national park, WY, USA
AU - Saini, Mohit Kumar
AU - Yoshida, Shohei
AU - Sebastian, Aswathy
AU - Hara, Eri
AU - Tamaki, Hideyuki
AU - Soulier, Nathan T.
AU - Albert, Istvan
AU - Hanada, Satoshi
AU - Tank, Marcus
AU - Bryant, Donald A.
N1 - Funding Information:
Studies in the laboratory of S.H. were funded by the Institute of Fermentation, Osaka (IFO), Japan. Studies in the laboratory of D.A.B. were supported by grant NNX16AJ62G from the NASA Exobiology program and by grant DE-FG02-94ER20137 from the Photosynthetic Systems Program, Division of Chemical Sciences, Geosciences, and Biosciences (CSGB), Office of Basic Energy Sciences of the U.S. Department of Energy. M.K.S. would like to thank Tokyo Metropolitan Government, Tokyo, Japan for support via a “Tokyo Human Resources Fund for City Diplomacy” scholarship.The authors gratefully acknowledge Craig Praul and his associates for their excellent technical assistance in the sequencing of this strain. The authors also gratefully acknowledge David M. Ward, Montana State University, for his assistance in field collections of mat samples. This study was conducted under Yellowstone National Park permit YELL-0129 to David M. Ward. Finally, the authors acknowledge the support and assistance of National Park Service Personnel at Yellowstone National Park.
Funding Information:
Funding: Studies in the laboratory of S.H. were funded by the Institute of Fermentation, Osaka (IFO), Japan. Studies in the laboratory of D.A.B. were supported by grant NNX16AJ62G from the NASA Exobiology program and by grant DE-FG02-94ER20137 from the Photosynthetic Systems Program, Division of Chemical Sciences, Geosciences, and Biosciences (CSGB), Office of Basic Energy Sciences of the U.S. Department of Energy. M.K.S. would like to thank Tokyo Metropolitan Government, Tokyo, Japan for support via a “Tokyo Human Resources Fund for City Diplomacy” scholarship.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
PY - 2022/1
Y1 - 2022/1
N2 - Strain MS-P2T was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth under strictly oxic conditions. Strain MS-P2T synthesizes bacteriochlorophyll a and the carotenoid spirilloxanthin. However, photoautotrophic growth did not occur under oxic or anoxic conditions, suggesting that this strain should be classified as an aerobic anoxygenic phototrophic bacterium. Strain MS-P2T cells are motile, curved rods about 0.5 to 1.0 µm wide and 1.0 to 1.5 µm long. The optimum growth temperature is 45–50◦C, and the optimum pH for growth is circum-neutral (pH 7.0–7.5). Sequence analysis of the 16S rRNA gene revealed that strain MS-P2T is closely related to Elioraea species, members of the class Alphaproteobacteria, with a sequence identity of 96.58 to 98%. The genome of strain MS-P2T is a single circular DNA molecule of 3,367,643 bp with a mol% guanine-plus-cytosine content of 70.6%. Based on phylogenetic, physiological, biochemical, and genomic characteristics, we propose this bacteriochlorophyll a-containing isolate is a new species belonging to the genus Elioraea, with the suggested name Elioraea tepida. The type-strain is strain MS-P2T (= JCM33060T = ATCC TSD-174T).
AB - Strain MS-P2T was isolated from microbial mats associated with Mushroom Spring, an alkaline siliceous hot spring in Yellowstone National Park, WY, USA. The isolate grows chemoheterotrophically by oxygen-dependent respiration, and light stimulates photoheterotrophic growth under strictly oxic conditions. Strain MS-P2T synthesizes bacteriochlorophyll a and the carotenoid spirilloxanthin. However, photoautotrophic growth did not occur under oxic or anoxic conditions, suggesting that this strain should be classified as an aerobic anoxygenic phototrophic bacterium. Strain MS-P2T cells are motile, curved rods about 0.5 to 1.0 µm wide and 1.0 to 1.5 µm long. The optimum growth temperature is 45–50◦C, and the optimum pH for growth is circum-neutral (pH 7.0–7.5). Sequence analysis of the 16S rRNA gene revealed that strain MS-P2T is closely related to Elioraea species, members of the class Alphaproteobacteria, with a sequence identity of 96.58 to 98%. The genome of strain MS-P2T is a single circular DNA molecule of 3,367,643 bp with a mol% guanine-plus-cytosine content of 70.6%. Based on phylogenetic, physiological, biochemical, and genomic characteristics, we propose this bacteriochlorophyll a-containing isolate is a new species belonging to the genus Elioraea, with the suggested name Elioraea tepida. The type-strain is strain MS-P2T (= JCM33060T = ATCC TSD-174T).
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U2 - 10.3390/microorganisms10010080
DO - 10.3390/microorganisms10010080
M3 - Article
C2 - 35056529
AN - SCOPUS:85122234440
SN - 2076-2607
VL - 10
JO - Microorganisms
JF - Microorganisms
IS - 1
M1 - 80
ER -