ELISA analysis of BACTEC® bottles for the earlier diagnosis of tuberculosis

L. N. Friedman, A. E. Filderman, T. G. D'Aquila, H. Y. Reynolds

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Enzyme-linked immunosorbent assays (ELISA) have enabled earlier identification of Mycobacterium tuberculosis (TB) in clinical settings by utilizing both TB antibody and antigen detection. We studied the sensitivity and specificity of ELISA detection of TB antigen by using a commercially available anti-BCG antibody in conjunction with BACTEC® 7H12B culture bottles. We compared these results against those obtained with cultures of Mycobacterium avium-intracellulare and Mycobacterium kansasii. All BACTEC® bottles were inoculated with known concentrations of organisms. TB antigen was detected by ELISA in BACTEC® culture bottles of TB 12 days before the BACTEC® system could itself identity the species. A growth index of greater than or equal to 10 reliably indicated that tuberculosis antigen was detectable by ELISA. The correlation between growth index and antigen concentration was extremely high (r = 0.95 and p < 0.001). There was insignificant cross-reactivity with the other atypical mycobacteria at the levels of growth tested. This technique potentially offers a sensitive and specific means for the early identification of TB is BACTEC® culture bottles.

Original languageEnglish (US)
Pages (from-to)668-671
Number of pages4
JournalAmerican Review of Respiratory Disease
Volume140
Issue number3 I
DOIs
StatePublished - Jan 1 1989

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Early Diagnosis
Tuberculosis
Enzyme-Linked Immunosorbent Assay
Antigens
Growth
Mycobacterium kansasii
Mycobacterium avium Complex
Nontuberculous Mycobacteria
Mycobacterium bovis
Mycobacterium tuberculosis
Anti-Idiotypic Antibodies
Sensitivity and Specificity
Antibodies

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Friedman, L. N. ; Filderman, A. E. ; D'Aquila, T. G. ; Reynolds, H. Y. / ELISA analysis of BACTEC® bottles for the earlier diagnosis of tuberculosis. In: American Review of Respiratory Disease. 1989 ; Vol. 140, No. 3 I. pp. 668-671.
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abstract = "Enzyme-linked immunosorbent assays (ELISA) have enabled earlier identification of Mycobacterium tuberculosis (TB) in clinical settings by utilizing both TB antibody and antigen detection. We studied the sensitivity and specificity of ELISA detection of TB antigen by using a commercially available anti-BCG antibody in conjunction with BACTEC{\circledR} 7H12B culture bottles. We compared these results against those obtained with cultures of Mycobacterium avium-intracellulare and Mycobacterium kansasii. All BACTEC{\circledR} bottles were inoculated with known concentrations of organisms. TB antigen was detected by ELISA in BACTEC{\circledR} culture bottles of TB 12 days before the BACTEC{\circledR} system could itself identity the species. A growth index of greater than or equal to 10 reliably indicated that tuberculosis antigen was detectable by ELISA. The correlation between growth index and antigen concentration was extremely high (r = 0.95 and p < 0.001). There was insignificant cross-reactivity with the other atypical mycobacteria at the levels of growth tested. This technique potentially offers a sensitive and specific means for the early identification of TB is BACTEC{\circledR} culture bottles.",
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ELISA analysis of BACTEC® bottles for the earlier diagnosis of tuberculosis. / Friedman, L. N.; Filderman, A. E.; D'Aquila, T. G.; Reynolds, H. Y.

In: American Review of Respiratory Disease, Vol. 140, No. 3 I, 01.01.1989, p. 668-671.

Research output: Contribution to journalArticle

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