Embryonic gene expression and pro-protein processing of proSAAS during rodent development

Daniel J. Morgan, Nino Mzhavia, Bonnie Peng, Hui Pan, Lakshmi A. Devi, John E. Pintar

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In vitro assays have demonstrated that peptides derived from the recently-identified proSAAS precursor inhibit prohormone convertase 1 (PC1) suggesting that this novel peptide may function as an endogenous inhibitor of PC1. To further understand the role of proSAAS in vivo, we have investigated the expression of proSAAS mRNA and processing of proSAAS during pre- and early postnatal rodent development. In situ hybridization showed that, by embryonic day 12.5 (e12.5) in the rat, proSAAS mRNA was present in essentially all differentiating neurons in the mantle layer of the myelencephalon, metencephalon, diencephalon, spinal cord and several sympathetic ganglia. During later stages of prenatal development, widespread proSAAS expression continues in post-mitotic neurons of both the CNS and PNS and begins in endocrine cells of the anterior and intermediate pituitary. Although pro-SAAS expression overlaps with PC1 in several regions, its overall expression pattern is significantly more extensive, suggesting that proSAAS may be multifunctional during development. Processed forms of proSAAS are present by at least mid-gestation with marked accumulation of two C-terminal forms, comprising the PC1 inhibitory fragment of proSAAS.

Original languageEnglish (US)
Pages (from-to)1454-1462
Number of pages9
JournalJournal of neurochemistry
Volume93
Issue number6
DOIs
StatePublished - Jun 1 2005

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Proprotein Convertase 1
Gene expression
Rodentia
Gene Expression
Processing
Neurons
Myelencephalon
Metencephalon
Proteins
Diencephalon
Messenger RNA
Peptides
Sympathetic Ganglia
Endocrine Cells
In Situ Hybridization
Rats
Assays
Spinal Cord
Cells
Pregnancy

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Morgan, Daniel J. ; Mzhavia, Nino ; Peng, Bonnie ; Pan, Hui ; Devi, Lakshmi A. ; Pintar, John E. / Embryonic gene expression and pro-protein processing of proSAAS during rodent development. In: Journal of neurochemistry. 2005 ; Vol. 93, No. 6. pp. 1454-1462.
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Embryonic gene expression and pro-protein processing of proSAAS during rodent development. / Morgan, Daniel J.; Mzhavia, Nino; Peng, Bonnie; Pan, Hui; Devi, Lakshmi A.; Pintar, John E.

In: Journal of neurochemistry, Vol. 93, No. 6, 01.06.2005, p. 1454-1462.

Research output: Contribution to journalArticle

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