Embryonic stem cell-based mapping of developmental transcriptional programs

Esteban O. Mazzoni, Shaun Mahony, Michelina Iacovino, Carolyn A. Morrison, George Mountoufaris, Michael Closser, Warren A. Whyte, Richard A. Young, Michael Kyba, David K. Gifford, Hynek Wichterle

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The study of developmentally regulated transcription factors by chromatin immunoprecipitation and deep sequencing (ChIP-seq) faces two major obstacles: availability of ChIP-grade antibodies and access to sufficient number of cells. We describe versatile genome-wide analysis of transcription-factor binding sites by combining directed differentiation of embryonic stem cells and inducible expression of tagged proteins. We demonstrate its utility by mapping DNA-binding sites of transcription factors involved in motor neuron specification.

Original languageEnglish (US)
Pages (from-to)1056-1060
Number of pages5
JournalNature methods
Volume8
Issue number12
DOIs
StatePublished - Dec 1 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Mazzoni, E. O., Mahony, S., Iacovino, M., Morrison, C. A., Mountoufaris, G., Closser, M., Whyte, W. A., Young, R. A., Kyba, M., Gifford, D. K., & Wichterle, H. (2011). Embryonic stem cell-based mapping of developmental transcriptional programs. Nature methods, 8(12), 1056-1060. https://doi.org/10.1038/nmeth.1775