Embryonic Stem Cells from Parthenotes

Jose B. Cibelli, Kerrianne Cunniff, Kent Vrana

Research output: Contribution to journalReview article

37 Citations (Scopus)

Abstract

While human embryonic stem cells (hESCs) hold tremendous therapeutic potential, they also create societal and ethical dilemmas. Adult and placental stem cells represent two alternatives to the hESC, but may have technical limitations. An additional alternative is the stem cell derived from parthenogenesis. Parthenogenesis is a reproductive mechanism that is common in lower organisms and produces a live birth from an oocyte activated in the absence of sperm. However, parthenogenetic embryos will develop to the blastocyst stage and so can serve as a source of embryonic stem cells. Parthenogenetic ESCs (pESCs) have been shown to have the properties of self-renewal and the capacity to generate cell derivatives from the three germ layers, confirmed by contributions to chimeric animals and/or teratoma formation when injected into SCID mice. Therefore, this mechanism for generating stem cells has the ethical advantage of not involving the destruction of viable embryos. Moreover, the cells do not involve the union of male and female and so genetic material will be derived exclusively from the female oocyte donor (with the attendant potential immunological advantages). This chapter describes the biology underlying parthenogenesis, as well as provides detailed technical considerations for the production of pESCs.

Original languageEnglish (US)
Pages (from-to)117-135
Number of pages19
JournalMethods in enzymology
Volume418
DOIs
StatePublished - Dec 1 2006

Fingerprint

Parthenogenesis
Embryonic Stem Cells
Stem cells
Oocytes
Stem Cells
Embryonic Structures
Germ Layers
Adult Stem Cells
SCID Mice
Teratoma
Live Birth
Blastocyst
Spermatozoa
Genes
Animals
Derivatives
Human Embryonic Stem Cells
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Cibelli, Jose B. ; Cunniff, Kerrianne ; Vrana, Kent. / Embryonic Stem Cells from Parthenotes. In: Methods in enzymology. 2006 ; Vol. 418. pp. 117-135.
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Embryonic Stem Cells from Parthenotes. / Cibelli, Jose B.; Cunniff, Kerrianne; Vrana, Kent.

In: Methods in enzymology, Vol. 418, 01.12.2006, p. 117-135.

Research output: Contribution to journalReview article

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