Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics

Marta Sobrinos-Sanguino, Silvia Zorrilla, Christine D. Keating, Begoña Monterroso, Germán Rivas

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

There is growing interest in analyzing the effect of microenvironments, which may be mimicked through liquid-liquid phase separation (LLPS), on the reactivity of biological macromolecules. We report the encapsulation by microfluidics of the division protein FtsZ and a LLPS system inside microdroplets and their conversion into permeable vesicles (allowing ligand uptake), with higher yield, homogeneity and biomolecular compatibility than those previously described.

Original languageEnglish (US)
Pages (from-to)4775-4778
Number of pages4
JournalChemical Communications
Volume53
Issue number35
DOIs
StatePublished - Jan 1 2017

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Membrane Lipids
Encapsulation
Microfluidics
Liquids
Phase separation
Macromolecules
Ligands
Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Sobrinos-Sanguino, Marta ; Zorrilla, Silvia ; Keating, Christine D. ; Monterroso, Begoña ; Rivas, Germán. / Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics. In: Chemical Communications. 2017 ; Vol. 53, No. 35. pp. 4775-4778.
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Encapsulation of a compartmentalized cytoplasm mimic within a lipid membrane by microfluidics. / Sobrinos-Sanguino, Marta; Zorrilla, Silvia; Keating, Christine D.; Monterroso, Begoña; Rivas, Germán.

In: Chemical Communications, Vol. 53, No. 35, 01.01.2017, p. 4775-4778.

Research output: Contribution to journalArticle

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