Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions

K. R. Matthews, B. M. Jayarao, A. J. Guidry, E. F. Erbe, W. P. Wergin, S. P. Oliver

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

Original languageEnglish (US)
Pages (from-to)361-367
Number of pages7
JournalVeterinary Microbiology
Volume39
Issue number3-4
DOIs
StatePublished - Apr 1994

Fingerprint

Streptococcus uberis
encapsulation
Streptococcus
Capsules
India
agar
blood
Agar
skim milk
blood serum
egg yolk
Freeze Etching
breasts
electron microscopy
pathogenicity
researchers
Egg Yolk
secretion
cattle
organisms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • veterinary(all)

Cite this

Matthews, K. R. ; Jayarao, B. M. ; Guidry, A. J. ; Erbe, E. F. ; Wergin, W. P. ; Oliver, S. P. / Encapsulation of Streptococcus uberis : Influence of storage and cultural conditions. In: Veterinary Microbiology. 1994 ; Vol. 39, No. 3-4. pp. 361-367.
@article{e80ce5e5386c41688a8391153df719ff,
title = "Encapsulation of Streptococcus uberis: Influence of storage and cultural conditions",
abstract = "Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10{\%} skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.",
author = "Matthews, {K. R.} and Jayarao, {B. M.} and Guidry, {A. J.} and Erbe, {E. F.} and Wergin, {W. P.} and Oliver, {S. P.}",
year = "1994",
month = "4",
doi = "10.1016/0378-1135(94)90171-6",
language = "English (US)",
volume = "39",
pages = "361--367",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

Encapsulation of Streptococcus uberis : Influence of storage and cultural conditions. / Matthews, K. R.; Jayarao, B. M.; Guidry, A. J.; Erbe, E. F.; Wergin, W. P.; Oliver, S. P.

In: Veterinary Microbiology, Vol. 39, No. 3-4, 04.1994, p. 361-367.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Encapsulation of Streptococcus uberis

T2 - Influence of storage and cultural conditions

AU - Matthews, K. R.

AU - Jayarao, B. M.

AU - Guidry, A. J.

AU - Erbe, E. F.

AU - Wergin, W. P.

AU - Oliver, S. P.

PY - 1994/4

Y1 - 1994/4

N2 - Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

AB - Streptococcus uberis (n = 100) isolated from bovine mammary secretions were assessed by India ink for expression of capsule. Organisms were evaluated under four conditions; (1) after primary culture on blood agar, (2) following 5 passages on blood agar, (3) after 5 passages in Trypticase Soy Broth (TSB), and (4) after storage in 10% skim milk. Strains from primary culture (44 of 100) were positive for an unstained halo (capsule) by the India ink method. Number of strains expressing capsule decreased greatly after passage and following storage. Freeze-etching followed by electron microscopy confirmed results of India ink preparations. Strains were also cultured in various media to determine influence of medium components on capsule expression. Todd-Hewitt medium supplemented with either serum or egg yolk enhanced the size of capsule expressed. Results of this study may aid researchers investigating the pathogenicity of S. uberis.

UR - http://www.scopus.com/inward/record.url?scp=0028268162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028268162&partnerID=8YFLogxK

U2 - 10.1016/0378-1135(94)90171-6

DO - 10.1016/0378-1135(94)90171-6

M3 - Article

C2 - 7518989

AN - SCOPUS:0028268162

VL - 39

SP - 361

EP - 367

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -