Abstract
Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 337-348 |
| Number of pages | 12 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 687 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 13 1996 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring. / Bringmann, Gerhard; Friedrich, Heike; Birner, Gerhard; Koob, Michael; Sontag, Karl Heinz; Heim, Christine; Kolasiewicz, Waclaw; Fähr, Silvia; Stäblein, Michaela; God, Ralf; Feineis, Doris.
In: Journal of Chromatography B: Biomedical Applications, Vol. 687, No. 2, 13.12.1996, p. 337-348.Research output: Contribution to journal › Article
TY - JOUR
T1 - Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring
AU - Bringmann, Gerhard
AU - Friedrich, Heike
AU - Birner, Gerhard
AU - Koob, Michael
AU - Sontag, Karl Heinz
AU - Heim, Christine
AU - Kolasiewicz, Waclaw
AU - Fähr, Silvia
AU - Stäblein, Michaela
AU - God, Ralf
AU - Feineis, Doris
PY - 1996/12/13
Y1 - 1996/12/13
N2 - Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.
AB - Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.
UR - http://www.scopus.com/inward/record.url?scp=0030582743&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030582743&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(96)00255-1
DO - 10.1016/S0378-4347(96)00255-1
M3 - Article
C2 - 9017456
AN - SCOPUS:0030582743
VL - 687
SP - 337
EP - 348
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 2
ER -