Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring

Gerhard Bringmann, Heike Friedrich, Gerhard Birner, Michael Koob, Karl Heinz Sontag, Christine Heim, Waclaw Kolasiewicz, Silvia Fähr, Michaela Stäblein, Ralf God, Doris Feineis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.

Original languageEnglish (US)
Pages (from-to)337-348
Number of pages12
JournalJournal of Chromatography B: Biomedical Applications
Volume687
Issue number2
DOIs
StatePublished - Dec 13 1996

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Carbolines
heptafluorobutyric anhydride
Neurotoxins
Alkaloids
Gas chromatography
Ions
Chloral Hydrate
Monitoring
Deuterium
Fused silica
Chromatography
Amides
Purification
Rats
Condensation
Assays
Brain
Blood
Gases
Tissue

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Bringmann, Gerhard ; Friedrich, Heike ; Birner, Gerhard ; Koob, Michael ; Sontag, Karl Heinz ; Heim, Christine ; Kolasiewicz, Waclaw ; Fähr, Silvia ; Stäblein, Michaela ; God, Ralf ; Feineis, Doris. / Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring. In: Journal of Chromatography B: Biomedical Applications. 1996 ; Vol. 687, No. 2. pp. 337-348.
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abstract = "Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.",
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Endogenous alkaloids in man XXVI. Determination of the dopaminergic neurotoxin 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo) in biological samples using gas chromatography with selected ion monitoring. / Bringmann, Gerhard; Friedrich, Heike; Birner, Gerhard; Koob, Michael; Sontag, Karl Heinz; Heim, Christine; Kolasiewicz, Waclaw; Fähr, Silvia; Stäblein, Michaela; God, Ralf; Feineis, Doris.

In: Journal of Chromatography B: Biomedical Applications, Vol. 687, No. 2, 13.12.1996, p. 337-348.

Research output: Contribution to journalArticle

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AU - Bringmann, Gerhard

AU - Friedrich, Heike

AU - Birner, Gerhard

AU - Koob, Michael

AU - Sontag, Karl Heinz

AU - Heim, Christine

AU - Kolasiewicz, Waclaw

AU - Fähr, Silvia

AU - Stäblein, Michaela

AU - God, Ralf

AU - Feineis, Doris

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AB - Highly chlorinated β-carbolines have a potential in vivo relevance to Parkinson's disease. In this paper, a gas chromatographic method for the determination of the neurotoxic 1-trichloromethyl-1,2,3,4-tetrahydro-β-carboline (TaClo), the condensation product of tryptamine and chloral hydrate, is described. The specific and sensitive assay involves purification of the biological samples by solid-phase extraction with C18 cartridges, derivatization with heptafluorobutyric anhydride, and chromatography on a non-polar fused-silica capillary column. Detection of TaClo was achieved by the registration of characteristic mass fragments of the TaClo heptafluorobutyric amide derivative using selected ion monitoring. The method was utilized to detect and quantify TaClo in blood, urine, bile, faeces, and brain tissue of rats treated with this alkaloid-type heterocycle. Four-fold deuterium-labelled TaClo was used as an internal standard.

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