Endogenous lectin from terminally differentiated epidermal cells

Miriam M. Brysk, Srinivasan Rajaraman, Philip Penn, Shu-jen Chen

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We isolated a concanavalin A (Con-A)-binding glycoprotein from human stratum corneum by nonionic detergent extraction, lectin affinity chromatography, and preparative gel electrophoresis. This glycoprotein migrates as a single band at 40 kilodaltons at sodium-dodecyl-sulfate gel electrophoresis with or without the presence of 2-mercaptoethanol. It was shown to have a heterogeneous distribution between pH 5.6 and 7.6 by isoelectric focusing. The glycoprotein is histidine rich (10.4%) but is distinct from other histidine-rich proteins (epidermal filaggrin and the histidine-rich glycoprotein from serum). It does not bind to lectins specific for L-fucose or α-D-galactose. We prepared a monospecific polyclonal antibody to the 40-kilodalton glycoprotein; at the ultrastructural level, it cytoimmunolocalizes exclusively to the membranes of the stratum corneum. A unique feature of the glycoprotein is that it is an endogenous lectin: it hemagglutinates trypsinized and gluteraldehyde-fixed rabbit erythrocytes. The inhibition of its hemagglutination was found to be greatest with amino sugars, down to a saccharide concentration of 10−5 mM. Such a high affinity of binding at the cell surface suggests that this glycoprotein is a major carbohydrate-binding, cross-linking molecule that holds adjacent corneocytes together in the stratum corneum. We hypothesize that this lectin plays a role in the adhesion and desquamation of the stratum corneum.

Original languageEnglish (US)
Pages (from-to)230-237
Number of pages8
JournalDifferentiation
Volume32
Issue number3
DOIs
StatePublished - Jan 1 1986

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Lectins
Cornea
Glycoproteins
Electrophoresis
Gels
Amino Sugars
Mercaptoethanol
Membrane Glycoproteins
Hemagglutination
Isoelectric Focusing
Galactose
Affinity Chromatography
Sodium Dodecyl Sulfate
Detergents
Erythrocytes
Carbohydrates
Rabbits
Membranes
Antibodies
Serum

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this

Brysk, Miriam M. ; Rajaraman, Srinivasan ; Penn, Philip ; Chen, Shu-jen. / Endogenous lectin from terminally differentiated epidermal cells. In: Differentiation. 1986 ; Vol. 32, No. 3. pp. 230-237.
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Endogenous lectin from terminally differentiated epidermal cells. / Brysk, Miriam M.; Rajaraman, Srinivasan; Penn, Philip; Chen, Shu-jen.

In: Differentiation, Vol. 32, No. 3, 01.01.1986, p. 230-237.

Research output: Contribution to journalArticle

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