Endogenous opioid systems and neural cancer: Transmission and scanning electron microscopic studies of murine neuroblastoma in tissue culture

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Endogenous opioid systems participate in carcinogenic events. To understand further the action of opioids on growth, S20Y neuroblastoma cells in tissue culture were exposed to i) [Met5]-enkephalin, a naturally occurring opioid pentapeptide, at a concentration (10-6 M) that inhibits cell replication by 66% of control levels, ii) [Met5]-enkephalin (10-6 M) and the opioid antagonist naloxone (10-6 M) which blocks opioid agonist action, or iii) naltrexone (10-6 M), a potent antagonist that disrupts endogenous opioid-opioid receptor interaction and increased cell number 76% above control values. The morphology of cells exposed to these agents for 2-4 days were similar to controls (i.e., exposed to sterile water) as determined by scanning and transmission electron microscopy. These results support the hypothesis that endogenous opioid systems act as trophic factors as they regulate growth; their effects on cell growth and survival, however, do not alter the basic ultrastructural morphology of the cells. Moreover, these data strengthen the validity of paradigms and therapeutic regimens that utilize opioid agonists and antagonists to modulate the relationship of endogenous opioid-opioid receptor interactions in neural cancer.

Original languageEnglish (US)
Pages (from-to)777-784
Number of pages8
JournalBrain Research Bulletin
Volume21
Issue number5
DOIs
StatePublished - Jan 1 1988

Fingerprint

Neuroblastoma
Synaptic Transmission
Opioid Analgesics
Electrons
Neoplasms
Narcotic Antagonists
Enkephalins
Opioid Receptors
Growth
Naltrexone
Scanning Transmission Electron Microscopy
Naloxone
Cell Survival
Cell Count
Water

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{f20a542611b2441084e2e7d3fd5a45f0,
title = "Endogenous opioid systems and neural cancer: Transmission and scanning electron microscopic studies of murine neuroblastoma in tissue culture",
abstract = "Endogenous opioid systems participate in carcinogenic events. To understand further the action of opioids on growth, S20Y neuroblastoma cells in tissue culture were exposed to i) [Met5]-enkephalin, a naturally occurring opioid pentapeptide, at a concentration (10-6 M) that inhibits cell replication by 66{\%} of control levels, ii) [Met5]-enkephalin (10-6 M) and the opioid antagonist naloxone (10-6 M) which blocks opioid agonist action, or iii) naltrexone (10-6 M), a potent antagonist that disrupts endogenous opioid-opioid receptor interaction and increased cell number 76{\%} above control values. The morphology of cells exposed to these agents for 2-4 days were similar to controls (i.e., exposed to sterile water) as determined by scanning and transmission electron microscopy. These results support the hypothesis that endogenous opioid systems act as trophic factors as they regulate growth; their effects on cell growth and survival, however, do not alter the basic ultrastructural morphology of the cells. Moreover, these data strengthen the validity of paradigms and therapeutic regimens that utilize opioid agonists and antagonists to modulate the relationship of endogenous opioid-opioid receptor interactions in neural cancer.",
author = "Ian Zagon",
year = "1988",
month = "1",
day = "1",
doi = "10.1016/0361-9230(88)90046-9",
language = "English (US)",
volume = "21",
pages = "777--784",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Endogenous opioid systems and neural cancer

T2 - Transmission and scanning electron microscopic studies of murine neuroblastoma in tissue culture

AU - Zagon, Ian

PY - 1988/1/1

Y1 - 1988/1/1

N2 - Endogenous opioid systems participate in carcinogenic events. To understand further the action of opioids on growth, S20Y neuroblastoma cells in tissue culture were exposed to i) [Met5]-enkephalin, a naturally occurring opioid pentapeptide, at a concentration (10-6 M) that inhibits cell replication by 66% of control levels, ii) [Met5]-enkephalin (10-6 M) and the opioid antagonist naloxone (10-6 M) which blocks opioid agonist action, or iii) naltrexone (10-6 M), a potent antagonist that disrupts endogenous opioid-opioid receptor interaction and increased cell number 76% above control values. The morphology of cells exposed to these agents for 2-4 days were similar to controls (i.e., exposed to sterile water) as determined by scanning and transmission electron microscopy. These results support the hypothesis that endogenous opioid systems act as trophic factors as they regulate growth; their effects on cell growth and survival, however, do not alter the basic ultrastructural morphology of the cells. Moreover, these data strengthen the validity of paradigms and therapeutic regimens that utilize opioid agonists and antagonists to modulate the relationship of endogenous opioid-opioid receptor interactions in neural cancer.

AB - Endogenous opioid systems participate in carcinogenic events. To understand further the action of opioids on growth, S20Y neuroblastoma cells in tissue culture were exposed to i) [Met5]-enkephalin, a naturally occurring opioid pentapeptide, at a concentration (10-6 M) that inhibits cell replication by 66% of control levels, ii) [Met5]-enkephalin (10-6 M) and the opioid antagonist naloxone (10-6 M) which blocks opioid agonist action, or iii) naltrexone (10-6 M), a potent antagonist that disrupts endogenous opioid-opioid receptor interaction and increased cell number 76% above control values. The morphology of cells exposed to these agents for 2-4 days were similar to controls (i.e., exposed to sterile water) as determined by scanning and transmission electron microscopy. These results support the hypothesis that endogenous opioid systems act as trophic factors as they regulate growth; their effects on cell growth and survival, however, do not alter the basic ultrastructural morphology of the cells. Moreover, these data strengthen the validity of paradigms and therapeutic regimens that utilize opioid agonists and antagonists to modulate the relationship of endogenous opioid-opioid receptor interactions in neural cancer.

UR - http://www.scopus.com/inward/record.url?scp=0024148934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024148934&partnerID=8YFLogxK

U2 - 10.1016/0361-9230(88)90046-9

DO - 10.1016/0361-9230(88)90046-9

M3 - Article

C2 - 3219609

AN - SCOPUS:0024148934

VL - 21

SP - 777

EP - 784

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 5

ER -