Endometrial Dye Instillation: A Novel Approach to Histopathologic Evaluation of Morcellated Hysterectomy Specimens

Teresa Tam, Gerald Harkins, Trevor Caldwell, Richard Zaino, Danielle Hazard

Research output: Contribution to journalArticle

5 Scopus citations


The purpose of this prospective pilot case study was to determine whether instillation of trypan blue dye into the uterine cavity before laparoscopic hysterectomy and morcellation aids in gross identification of endometrium. The most common commercially available trypan blue stain, VisionBlue was used in this study. Instillation was performed at the beginning of the procedure using an embryo transfer catheter. A sterile solution of trypan blue, 0.5 mL, was instilled transcervically into the uterine cavities in 12 patients before laparoscopic hysterectomy with uterine morcellation. The morcellated specimens were sent for routine gross pathologic and histologic examination. It was concluded that intrauterine instillation of trypan blue stained the endometrium, thus aiding the pathologist in identification of the endometrium in morcellated uterine specimens.

Original languageEnglish (US)
Pages (from-to)667-671
Number of pages5
JournalJournal of Minimally Invasive Gynecology
Issue number5
StatePublished - Sep 1 2013


All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

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