Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells

Boon Seng Soh, Shi Yan Ng, Hao Wu, Kristina Buac, Joo Hye C. Park, Xiaojun Lian, Jiejia Xu, Kylie S. Foo, Ulrika Felldin, Xiaobing He, Massimo Nichane, Henry Yang, Lei Bu, Ronald A. Li, Bing Lim, Kenneth R. Chien

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins. Progenitor clones undergo long-term expansion and differentiate primarily into endothelial and smooth muscle cell lineages in vitro, and contribute extensively to coronary-like vessels in vivo, forming a functional human-mouse chimeric circulatory system. Our study identifies EDN1 as a key factor towards the generation and clonal derivation of ISL1 + vascular intermediates, and demonstrates the intrinsic cell-autonomous nature of these progenitors to differentiate and self-organize into functional vasculatures in vivo.

Original languageEnglish (US)
Article number10774
JournalNature communications
Volume7
DOIs
StatePublished - Mar 8 2016

Fingerprint

stem cells
Endothelin-1
Stem cells
Blood Vessels
Muscle
smooth muscle
muscle cells
derivation
Cells
Smooth Muscle Myocytes
expansion
Endothelial cells
cells
circulatory system
Recombinant Proteins
Cell Lineage
Tissue
Cardiovascular System
muscles
Vascular Smooth Muscle

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Soh, Boon Seng ; Ng, Shi Yan ; Wu, Hao ; Buac, Kristina ; Park, Joo Hye C. ; Lian, Xiaojun ; Xu, Jiejia ; Foo, Kylie S. ; Felldin, Ulrika ; He, Xiaobing ; Nichane, Massimo ; Yang, Henry ; Bu, Lei ; Li, Ronald A. ; Lim, Bing ; Chien, Kenneth R. / Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells. In: Nature communications. 2016 ; Vol. 7.
@article{7a5f58b1436f44debd369029b094890b,
title = "Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells",
abstract = "Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins. Progenitor clones undergo long-term expansion and differentiate primarily into endothelial and smooth muscle cell lineages in vitro, and contribute extensively to coronary-like vessels in vivo, forming a functional human-mouse chimeric circulatory system. Our study identifies EDN1 as a key factor towards the generation and clonal derivation of ISL1 + vascular intermediates, and demonstrates the intrinsic cell-autonomous nature of these progenitors to differentiate and self-organize into functional vasculatures in vivo.",
author = "Soh, {Boon Seng} and Ng, {Shi Yan} and Hao Wu and Kristina Buac and Park, {Joo Hye C.} and Xiaojun Lian and Jiejia Xu and Foo, {Kylie S.} and Ulrika Felldin and Xiaobing He and Massimo Nichane and Henry Yang and Lei Bu and Li, {Ronald A.} and Bing Lim and Chien, {Kenneth R.}",
year = "2016",
month = "3",
day = "8",
doi = "10.1038/ncomms10774",
language = "English (US)",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

Soh, BS, Ng, SY, Wu, H, Buac, K, Park, JHC, Lian, X, Xu, J, Foo, KS, Felldin, U, He, X, Nichane, M, Yang, H, Bu, L, Li, RA, Lim, B & Chien, KR 2016, 'Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells', Nature communications, vol. 7, 10774. https://doi.org/10.1038/ncomms10774

Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells. / Soh, Boon Seng; Ng, Shi Yan; Wu, Hao; Buac, Kristina; Park, Joo Hye C.; Lian, Xiaojun; Xu, Jiejia; Foo, Kylie S.; Felldin, Ulrika; He, Xiaobing; Nichane, Massimo; Yang, Henry; Bu, Lei; Li, Ronald A.; Lim, Bing; Chien, Kenneth R.

In: Nature communications, Vol. 7, 10774, 08.03.2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells

AU - Soh, Boon Seng

AU - Ng, Shi Yan

AU - Wu, Hao

AU - Buac, Kristina

AU - Park, Joo Hye C.

AU - Lian, Xiaojun

AU - Xu, Jiejia

AU - Foo, Kylie S.

AU - Felldin, Ulrika

AU - He, Xiaobing

AU - Nichane, Massimo

AU - Yang, Henry

AU - Bu, Lei

AU - Li, Ronald A.

AU - Lim, Bing

AU - Chien, Kenneth R.

PY - 2016/3/8

Y1 - 2016/3/8

N2 - Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins. Progenitor clones undergo long-term expansion and differentiate primarily into endothelial and smooth muscle cell lineages in vitro, and contribute extensively to coronary-like vessels in vivo, forming a functional human-mouse chimeric circulatory system. Our study identifies EDN1 as a key factor towards the generation and clonal derivation of ISL1 + vascular intermediates, and demonstrates the intrinsic cell-autonomous nature of these progenitors to differentiate and self-organize into functional vasculatures in vivo.

AB - Coronary arteriogenesis is a central step in cardiogenesis, requiring coordinated generation and integration of endothelial cell and vascular smooth muscle cells. At present, it is unclear whether the cell fate programme of cardiac progenitors to generate complex muscular or vascular structures is entirely cell autonomous. Here we demonstrate the intrinsic ability of vascular progenitors to develop and self-organize into cardiac tissues by clonally isolating and expanding second heart field cardiovascular progenitors using WNT3A and endothelin-1 (EDN1) human recombinant proteins. Progenitor clones undergo long-term expansion and differentiate primarily into endothelial and smooth muscle cell lineages in vitro, and contribute extensively to coronary-like vessels in vivo, forming a functional human-mouse chimeric circulatory system. Our study identifies EDN1 as a key factor towards the generation and clonal derivation of ISL1 + vascular intermediates, and demonstrates the intrinsic cell-autonomous nature of these progenitors to differentiate and self-organize into functional vasculatures in vivo.

UR - http://www.scopus.com/inward/record.url?scp=84960391169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960391169&partnerID=8YFLogxK

U2 - 10.1038/ncomms10774

DO - 10.1038/ncomms10774

M3 - Article

C2 - 26952167

AN - SCOPUS:84960391169

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 10774

ER -