Endotoxin-induced alterations in insulin-stimulated phosphorylation of insulin receptor, IRS-1, and map kinase in skeletal muscle

Jie Fan, Yue Hua Li, Margaret M. Wojnar, Charles H. Lang

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Sepsis and endotoxin (LPS) have been demonstrated to impair insulin-mediated glucose uptake in skeletal muscle. However, the intracellular mechanism responsible for this defect is not fully defined. The purpose of the present study was to determine whether specific elements of the insulin receptor (IR) signaling pathway in skeletal muscle are altered by LPS. In vivo injection of Escherichia coli LPS resulted in a 44% reduction in whole body glucose disposal under euglycemic hyperinsulinemic conditions, which was largely accounted for by a decreased rate of glycogen synthesis. Scatchard analysis indicated that the number and affinity of the high-affinity insulin binding sites in muscle were similar between control and LPS-treated rats. Western blot analysis indicated that under basal conditions, the levels of total and phosphorylated IR, insulin receptor substrate (IRS)-1, and mitogen-activated protein (MAP) kinase were not significantly different between control and endotoxic rats. In control animals, muscle obtained 2 min after intravenous injection of a maximally stimulating dose of insulin demonstrated a marked increase in the amount of phosphorylated IR (∼5-fold), IRS-1 (∼10-fold), and MAP kinase (∼10-fold). Insulin-stimulated phosphorylation of IR, IRS-1, and MAP kinase was markedly diminished (∼75%, 90%, and 78%, respectively) in LPS-treated rats. However, there was no concomitant reduction in the total abundance of these proteins under hyperinsulinemic conditions. These data demonstrate that LPS alters multiple steps in the insulin signal transduction pathway, but not insulin binding, in skeletal muscle that may mediate the observed impairment in glucose uptake.

Original languageEnglish (US)
Pages (from-to)164-170
Number of pages7
JournalShock
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1996

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Insulin Receptor Substrate Proteins
Insulin Receptor
Endotoxins
Skeletal Muscle
Phosphotransferases
Phosphorylation
Insulin
Mitogen-Activated Protein Kinases
Glucose
Mitogen-Activated Protein Kinase 10
Muscles
Glycogen
Intravenous Injections
Signal Transduction
Sepsis
Western Blotting
Binding Sites
Escherichia coli
Injections
Proteins

All Science Journal Classification (ASJC) codes

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

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title = "Endotoxin-induced alterations in insulin-stimulated phosphorylation of insulin receptor, IRS-1, and map kinase in skeletal muscle",
abstract = "Sepsis and endotoxin (LPS) have been demonstrated to impair insulin-mediated glucose uptake in skeletal muscle. However, the intracellular mechanism responsible for this defect is not fully defined. The purpose of the present study was to determine whether specific elements of the insulin receptor (IR) signaling pathway in skeletal muscle are altered by LPS. In vivo injection of Escherichia coli LPS resulted in a 44{\%} reduction in whole body glucose disposal under euglycemic hyperinsulinemic conditions, which was largely accounted for by a decreased rate of glycogen synthesis. Scatchard analysis indicated that the number and affinity of the high-affinity insulin binding sites in muscle were similar between control and LPS-treated rats. Western blot analysis indicated that under basal conditions, the levels of total and phosphorylated IR, insulin receptor substrate (IRS)-1, and mitogen-activated protein (MAP) kinase were not significantly different between control and endotoxic rats. In control animals, muscle obtained 2 min after intravenous injection of a maximally stimulating dose of insulin demonstrated a marked increase in the amount of phosphorylated IR (∼5-fold), IRS-1 (∼10-fold), and MAP kinase (∼10-fold). Insulin-stimulated phosphorylation of IR, IRS-1, and MAP kinase was markedly diminished (∼75{\%}, 90{\%}, and 78{\%}, respectively) in LPS-treated rats. However, there was no concomitant reduction in the total abundance of these proteins under hyperinsulinemic conditions. These data demonstrate that LPS alters multiple steps in the insulin signal transduction pathway, but not insulin binding, in skeletal muscle that may mediate the observed impairment in glucose uptake.",
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Endotoxin-induced alterations in insulin-stimulated phosphorylation of insulin receptor, IRS-1, and map kinase in skeletal muscle. / Fan, Jie; Li, Yue Hua; Wojnar, Margaret M.; Lang, Charles H.

In: Shock, Vol. 6, No. 3, 01.01.1996, p. 164-170.

Research output: Contribution to journalArticle

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