Energy metabolism in isolated chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle at various temperatures in vitro

E. Y. Suzuki, R. J. Early, Paul H. Patterson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Muscle respiration experiments on inhibitor dosage (experiment 1), muscle preparation (tendons removed vs. unstretched vs. stretched muscles; chick muscle only; experiment 2) and media temperature (26.5, 32, 37, 42°C; experiment 3) were conducted on chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle in vitro. Experiment 1: The dosage of cycloheximide and ouabain required for maximum inhibition of protein synthesis and Na+,K+ ATPase, respectively, in chick and tilapia muscle was approximately 6 × 10-5 M. Experiment 2: Removing the tendons of chick muscle decreased (% inhibition, P = 0.05) cycloheximide-sensitive respiration compared to stretched and unstretched muscles (tendons intact). However, muscle preparation had little influence on ouabain-sensitive respiration. Experiment 3: Cycloheximide-sensitive respiration tended to increase (μ O2/mg DNA -fhr, P = 0.054) with media temperature in tilapia muscle. Chick muscle was less responsive in this respect. Ouabain-sensitive respiration increased at lower temperature in chick muscle (% inhibition, cubic relationship, P = 0.001) and at higher temperature in tilapia muscle (% inhibition, quadratic relationship, P = 0.0002).

Original languageEnglish (US)
Pages (from-to)139-150
Number of pages12
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume109
Issue number1
DOIs
StatePublished - Jan 1 1994

Fingerprint

Tilapia
Energy Metabolism
Chickens
Muscles
Temperature
Respiration
Ouabain
Cycloheximide
Tendons
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

@article{ccbd5f9ac9b348f195056860cfa13d5f,
title = "Energy metabolism in isolated chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle at various temperatures in vitro",
abstract = "Muscle respiration experiments on inhibitor dosage (experiment 1), muscle preparation (tendons removed vs. unstretched vs. stretched muscles; chick muscle only; experiment 2) and media temperature (26.5, 32, 37, 42°C; experiment 3) were conducted on chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle in vitro. Experiment 1: The dosage of cycloheximide and ouabain required for maximum inhibition of protein synthesis and Na+,K+ ATPase, respectively, in chick and tilapia muscle was approximately 6 × 10-5 M. Experiment 2: Removing the tendons of chick muscle decreased ({\%} inhibition, P = 0.05) cycloheximide-sensitive respiration compared to stretched and unstretched muscles (tendons intact). However, muscle preparation had little influence on ouabain-sensitive respiration. Experiment 3: Cycloheximide-sensitive respiration tended to increase (μ O2/mg DNA -fhr, P = 0.054) with media temperature in tilapia muscle. Chick muscle was less responsive in this respect. Ouabain-sensitive respiration increased at lower temperature in chick muscle ({\%} inhibition, cubic relationship, P = 0.001) and at higher temperature in tilapia muscle ({\%} inhibition, quadratic relationship, P = 0.0002).",
author = "Suzuki, {E. Y.} and Early, {R. J.} and Patterson, {Paul H.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1016/0300-9629(94)90320-4",
language = "English (US)",
volume = "109",
pages = "139--150",
journal = "Comparative Biochemistry and Physiology -- Part A: Physiology",
issn = "0300-9629",
number = "1",

}

TY - JOUR

T1 - Energy metabolism in isolated chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle at various temperatures in vitro

AU - Suzuki, E. Y.

AU - Early, R. J.

AU - Patterson, Paul H.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Muscle respiration experiments on inhibitor dosage (experiment 1), muscle preparation (tendons removed vs. unstretched vs. stretched muscles; chick muscle only; experiment 2) and media temperature (26.5, 32, 37, 42°C; experiment 3) were conducted on chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle in vitro. Experiment 1: The dosage of cycloheximide and ouabain required for maximum inhibition of protein synthesis and Na+,K+ ATPase, respectively, in chick and tilapia muscle was approximately 6 × 10-5 M. Experiment 2: Removing the tendons of chick muscle decreased (% inhibition, P = 0.05) cycloheximide-sensitive respiration compared to stretched and unstretched muscles (tendons intact). However, muscle preparation had little influence on ouabain-sensitive respiration. Experiment 3: Cycloheximide-sensitive respiration tended to increase (μ O2/mg DNA -fhr, P = 0.054) with media temperature in tilapia muscle. Chick muscle was less responsive in this respect. Ouabain-sensitive respiration increased at lower temperature in chick muscle (% inhibition, cubic relationship, P = 0.001) and at higher temperature in tilapia muscle (% inhibition, quadratic relationship, P = 0.0002).

AB - Muscle respiration experiments on inhibitor dosage (experiment 1), muscle preparation (tendons removed vs. unstretched vs. stretched muscles; chick muscle only; experiment 2) and media temperature (26.5, 32, 37, 42°C; experiment 3) were conducted on chick (Gallus domesticus) gastrocnemius and tilapia (Tilapia mossambica) epaxial muscle in vitro. Experiment 1: The dosage of cycloheximide and ouabain required for maximum inhibition of protein synthesis and Na+,K+ ATPase, respectively, in chick and tilapia muscle was approximately 6 × 10-5 M. Experiment 2: Removing the tendons of chick muscle decreased (% inhibition, P = 0.05) cycloheximide-sensitive respiration compared to stretched and unstretched muscles (tendons intact). However, muscle preparation had little influence on ouabain-sensitive respiration. Experiment 3: Cycloheximide-sensitive respiration tended to increase (μ O2/mg DNA -fhr, P = 0.054) with media temperature in tilapia muscle. Chick muscle was less responsive in this respect. Ouabain-sensitive respiration increased at lower temperature in chick muscle (% inhibition, cubic relationship, P = 0.001) and at higher temperature in tilapia muscle (% inhibition, quadratic relationship, P = 0.0002).

UR - http://www.scopus.com/inward/record.url?scp=0027964613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027964613&partnerID=8YFLogxK

U2 - 10.1016/0300-9629(94)90320-4

DO - 10.1016/0300-9629(94)90320-4

M3 - Article

VL - 109

SP - 139

EP - 150

JO - Comparative Biochemistry and Physiology -- Part A: Physiology

JF - Comparative Biochemistry and Physiology -- Part A: Physiology

SN - 0300-9629

IS - 1

ER -