Engineering bacterial signals and sensors

Howard Salis, Alvin Tamsir, Christopher Voigt

Research output: Chapter in Book/Report/Conference proceedingChapter

37 Scopus citations

Abstract

In the emerging field of synthetic biology, a central goal is to reliably engineer bacteria to respond to environmental signals according to a pre-determined genetic program. The sensor systems and genetic circuitry inside bacteria are the 'eyes' and 'brain' of a new class of biotechnological applications in which bacteria are used as living, self-replicating computers that can beneficially interact with the physical world. These engineered gene networks are constructed by extracting natural sensor systems and other genetic parts from multiple organisms and recombining them into novel configurations. This chapter is a how-to guide. It describes several strategies for engineering new bacterial sensor systems and synthetic gene networks that are capable of sensing a desired stimulus and generating interesting dynamical or pattern-forming responses. We also provide specification sheets describing many two-component and quorum-sensing systems, focusing on the information that one needs to know in order to use them for engineering applications.

Original languageEnglish (US)
Title of host publicationBacterial Sensing and Signaling
EditorsMattias Collin, Raymond Schuch
Pages194-225
Number of pages32
DOIs
StatePublished - 2009

Publication series

NameContributions to Microbiology
Volume16
ISSN (Print)1420-9519
ISSN (Electronic)1662-291X

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

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  • Cite this

    Salis, H., Tamsir, A., & Voigt, C. (2009). Engineering bacterial signals and sensors. In M. Collin, & R. Schuch (Eds.), Bacterial Sensing and Signaling (pp. 194-225). (Contributions to Microbiology; Vol. 16). https://doi.org/10.1159/000219381