Engineering enzymes for oxidizing nitrobenzene

Research output: Contribution to specialist publicationArticle

Abstract

Researchers at the University of Connecticut used DNA shuffling and saturation mutagenesis to generate random mutants as a way to increase the oxidation rate and change the oxidation regiospecificity of nitro aromatics. Improved mutants were successfully produced. One of them produced 4-nitrocatechol 4.5-fold faster and another 20-fold faster than the wild type enzyme. Another mutant changed the regiospecificity of nitrobenzene from 28% of p-nitrophenol to 79%. Manipulation of the enzymes created an enzyme that produces 37% 4-nitrocatechol and 63% nitrohydroquinone from m-nitrophenol, while the original enzyme produced only 4-nitrocatechol. Still another variant formed p-cresol as the major product (93%) from toluene.

Original languageEnglish (US)
Number of pages1
Volume27
No2
Specialist publicationIndustrial Bioprocessing
StatePublished - Feb 1 2005

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Chemical Engineering(all)
  • Organic Chemistry

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