Engineering enzymes for oxidizing nitrobenzene

Research output: Contribution to specialist publicationArticle

Abstract

Researchers at the University of Connecticut used DNA shuffling and saturation mutagenesis to generate random mutants as a way to increase the oxidation rate and change the oxidation regiospecificity of nitro aromatics. Improved mutants were successfully produced. One of them produced 4-nitrocatechol 4.5-fold faster and another 20-fold faster than the wild type enzyme. Another mutant changed the regiospecificity of nitrobenzene from 28% of p-nitrophenol to 79%. Manipulation of the enzymes created an enzyme that produces 37% 4-nitrocatechol and 63% nitrohydroquinone from m-nitrophenol, while the original enzyme produced only 4-nitrocatechol. Still another variant formed p-cresol as the major product (93%) from toluene.

Original languageEnglish (US)
Pages4
Number of pages1
Volume27
No2
Specialist publicationIndustrial Bioprocessing
StatePublished - Feb 2005

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Nitrobenzene
Enzymes
Oxidation
Mutagenesis
Toluene
DNA
Nitrophenols

All Science Journal Classification (ASJC) codes

  • Energy (miscellaneous)

Cite this

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Engineering enzymes for oxidizing nitrobenzene. / Wood, Thomas Keith.

In: Industrial Bioprocessing, Vol. 27, No. 2, 02.2005, p. 4.

Research output: Contribution to specialist publicationArticle

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