Engineering erythrocytes to be erythrosensors: First steps

Mark A. Milanick, Sarah Ritter, Kenith Meissner

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Molecules can be loaded into mammalian erythrocytes through a reversible lysis pore that forms in the membrane when placed in hypotonic media, the result being resealed red cell ghosts. Many studies on the sidedness of transport processes have utilized this approach. In addition, red cell ghosts encapsulated with enzymes have been used in patients to treat specific enzyme deficiencies, particularly when the substrate can cross the red cell membrane. Our long-term goal is to put fluorescent sensors inside erythrocytes, return the loaded red cell ghosts to the animal or patient, and then monitor the fluorescence non-invasively to follow changes in plasma analyte concentration. In this paper, we present a novel dialysis method for making the red cell ghosts. In addition, we present a theoretical analysis showing that it is not necessary that every loaded red cell ghost has the same dye concentration. Finally we discuss the constraints on the optimal affinity for the sensor/analyte interaction.

Original languageEnglish (US)
Pages (from-to)100-106
Number of pages7
JournalBlood Cells, Molecules, and Diseases
Volume47
Issue number2
DOIs
StatePublished - Aug 15 2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

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