Enhanced human lysozyme production in biofilm reactor by Kluyveromyces lactis K7

Duygu Ercan, Ali Demirci

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Lysozyme is an antimicrobial compound, which has been used in pharmaceutical and food industries. Chicken egg is the commercial source of lysozyme. However, human lysozyme is more effective and safer than egg-white lysozyme. Human milk is an important source for human lysozyme, but it is not feasible to provide the needed lysozyme commercially. Biofilm reactors provide passive immobilization of cells onto the solid support, which may lead to higher productivity. The aim was to evaluate the fermentation medium composition for enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor with plastic composite supports. Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor. Moreover, inhibition effect of NaCl and NH4Cl at the concentrations of 25 and 50mM was observed. Three factors Box-Behnken response surface design was conducted and the results suggested 16.3% lactose, 1.2% casamino acid, 0.8% yeast nitrogen base as optimum medium composition for maximum human lysozyme production. Overall, the human lysozyme production by K. lactis K7 was increased to 173U/ml, which is about 23% improvement in biofilm reactor and 57% improvement compared to the suspended-cell fermentation.

Original languageEnglish (US)
Pages (from-to)2-8
Number of pages7
JournalBiochemical Engineering Journal
Volume92
DOIs
StatePublished - Nov 5 2014

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Kluyveromyces
Biofilms
Muramidase
Enzymes
Yeast
Nitrogen
Yeasts
Fermentation
Egg White
Food Industry
Drug Industry
Human Milk
Lactose
Chemical analysis
Immobilization
Drug products
Plastics
Zea mays
Ovum
Chickens

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Enhanced human lysozyme production in biofilm reactor by Kluyveromyces lactis K7",
abstract = "Lysozyme is an antimicrobial compound, which has been used in pharmaceutical and food industries. Chicken egg is the commercial source of lysozyme. However, human lysozyme is more effective and safer than egg-white lysozyme. Human milk is an important source for human lysozyme, but it is not feasible to provide the needed lysozyme commercially. Biofilm reactors provide passive immobilization of cells onto the solid support, which may lead to higher productivity. The aim was to evaluate the fermentation medium composition for enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor with plastic composite supports. Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor. Moreover, inhibition effect of NaCl and NH4Cl at the concentrations of 25 and 50mM was observed. Three factors Box-Behnken response surface design was conducted and the results suggested 16.3{\%} lactose, 1.2{\%} casamino acid, 0.8{\%} yeast nitrogen base as optimum medium composition for maximum human lysozyme production. Overall, the human lysozyme production by K. lactis K7 was increased to 173U/ml, which is about 23{\%} improvement in biofilm reactor and 57{\%} improvement compared to the suspended-cell fermentation.",
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Enhanced human lysozyme production in biofilm reactor by Kluyveromyces lactis K7. / Ercan, Duygu; Demirci, Ali.

In: Biochemical Engineering Journal, Vol. 92, 05.11.2014, p. 2-8.

Research output: Contribution to journalArticle

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AU - Demirci, Ali

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AB - Lysozyme is an antimicrobial compound, which has been used in pharmaceutical and food industries. Chicken egg is the commercial source of lysozyme. However, human lysozyme is more effective and safer than egg-white lysozyme. Human milk is an important source for human lysozyme, but it is not feasible to provide the needed lysozyme commercially. Biofilm reactors provide passive immobilization of cells onto the solid support, which may lead to higher productivity. The aim was to evaluate the fermentation medium composition for enhanced human lysozyme production by Kluyveromyces lactis K7 in biofilm reactor with plastic composite supports. Yeast nitrogen base was selected as the best nitrogen source when compared to the yeast extract and corn steep liquor. Moreover, inhibition effect of NaCl and NH4Cl at the concentrations of 25 and 50mM was observed. Three factors Box-Behnken response surface design was conducted and the results suggested 16.3% lactose, 1.2% casamino acid, 0.8% yeast nitrogen base as optimum medium composition for maximum human lysozyme production. Overall, the human lysozyme production by K. lactis K7 was increased to 173U/ml, which is about 23% improvement in biofilm reactor and 57% improvement compared to the suspended-cell fermentation.

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