Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli

Toshinari Maeda, Viviana Sanchez-Torres, Thomas Keith Wood

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

To utilize fermentative bacteria for producing the alternative fuel hydrogen, we performed successive rounds of P1 transduction from the Keio Escherichia coli K-12 library to introduce multiple, stable mutations into a single bacterium to direct the metabolic flux toward hydrogen production. E. coli cells convert glucose to various organic acids (such as succinate, pyruvate, lactate, formate, and acetate) to synthesize energy and hydrogen from formate by the formate hydrogen-lyase (FHL) system that consists of hydrogenase 3 and formate dehydrogenase-H. We altered the regulation of FHL by inactivating the repressor encoded by hycA and by overexpressing the activator encoded by fhlA, removed hydrogen uptake activity by deleting hyaB (hydrogenase 1) and hybC (hydrogenase 2), redirected glucose metabolism to formate by using the fdnG, fdoG, narG, focA, focB, poxB, and aceE mutations, and inactivated the succinate and lactate synthesis pathways by deleting frdC and ldhA, respectively. The best of the metabolically engineered strains, BW25113 hyaB hybC hycA fdoG frdC ldhA aceE, increased hydrogen production 4.6-fold from glucose and increased the hydrogen yield twofold from 0.65 to 1.3 mol H2/mol glucose (maximum, 2 mol H2/mol glucose).

Original languageEnglish (US)
Pages (from-to)879-890
Number of pages12
JournalApplied Microbiology and Biotechnology
Volume77
Issue number4
DOIs
StatePublished - Dec 1 2007

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formic acid
Hydrogen
Escherichia coli
Hydrogenase
Glucose
Succinic Acid
Lactic Acid
Bacteria
Mutation
Pyruvic Acid
Libraries
Acetates
Acids
formate hydrogenlyase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "To utilize fermentative bacteria for producing the alternative fuel hydrogen, we performed successive rounds of P1 transduction from the Keio Escherichia coli K-12 library to introduce multiple, stable mutations into a single bacterium to direct the metabolic flux toward hydrogen production. E. coli cells convert glucose to various organic acids (such as succinate, pyruvate, lactate, formate, and acetate) to synthesize energy and hydrogen from formate by the formate hydrogen-lyase (FHL) system that consists of hydrogenase 3 and formate dehydrogenase-H. We altered the regulation of FHL by inactivating the repressor encoded by hycA and by overexpressing the activator encoded by fhlA, removed hydrogen uptake activity by deleting hyaB (hydrogenase 1) and hybC (hydrogenase 2), redirected glucose metabolism to formate by using the fdnG, fdoG, narG, focA, focB, poxB, and aceE mutations, and inactivated the succinate and lactate synthesis pathways by deleting frdC and ldhA, respectively. The best of the metabolically engineered strains, BW25113 hyaB hybC hycA fdoG frdC ldhA aceE, increased hydrogen production 4.6-fold from glucose and increased the hydrogen yield twofold from 0.65 to 1.3 mol H2/mol glucose (maximum, 2 mol H2/mol glucose).",
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Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli. / Maeda, Toshinari; Sanchez-Torres, Viviana; Wood, Thomas Keith.

In: Applied Microbiology and Biotechnology, Vol. 77, No. 4, 01.12.2007, p. 879-890.

Research output: Contribution to journalArticle

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