Enhanced mechanical properties of bacterial cellulose nanocomposites produced by co-culturing Gluconacetobacter hansenii and Escherichia coli under static conditions

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Including additives in the culture media during bacterial cellulose (BC)biosynthesis is a traditional method to produce BC-based nanocomposites. This study examines a novel fermentation process, which is to co-culture Gluconacetobacter hansenii (G. hansenii)with Escherichia coli (E. coli)under static conditions, to produce BC pellicles with enhanced mechanical properties. The mannose-rich exopolysaccharides (EPS)synthesized by E. coli were incorporated into the BC network and affected the aggregation of co-crystallized microfibrils without significantly changing the crystal sizes of BC. When co-culturing G. hansenii ATCC 23769 with E. coli ATCC 700728, which produced a low concentration of EPS at 3.3 ± 0.7 mg/L, the BC pellicles exhibited a Young's modulus of 4,874 ± 1144 MPa and a stress at break of 80.7 ± 21.1 MPa, which are 81.9% and 79.3% higher than those of pure BC, respectively. The growth dynamics of the two co-cultured strains suggested that the production of BC and EPS were enhanced through co-culturing fermentation.

Original languageEnglish (US)
Pages (from-to)12-20
Number of pages9
JournalCarbohydrate Polymers
Volume219
DOIs
StatePublished - Sep 1 2019

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Enhanced mechanical properties of bacterial cellulose nanocomposites produced by co-culturing Gluconacetobacter hansenii and Escherichia coli under static conditions'. Together they form a unique fingerprint.

Cite this