Enhanced production of bacterial cellulose under agitated condition and its material property analysis

Kuan Chen Cheng, Jeffrey Catchmark, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Bacterial cellulose (BC) demonstrates unique properties including high mechanical strength, high crystallinity, and high water retention ability, which make it an useful material and has been applied in many industries, such as food, paper manufacturing, and pharmaceutical application. In this study, different additives including agar, carboxymethylcellulose (CMC), microcrystalline cellulose, and sodium alginate were added into fermentation medium in agitated culture to enhance BC production by Acetobacter xylinum. The optimal additive was chosen based on the amount of BC produced. The produced BC were analyzed by using X-ray diffractometer (XRD), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TGA), and dynamic mechanical analysis (DMA). Among the evaluated additives, CMC yielded highest BC production (8.2 g/L) compared to the control (1.3 g/L). The results also indicated that CMC-altered BC production increased with CMC addition and reached saturation around 1%. The variation between replicates for all analysis was less than 5%. From XRD pattern, however, the crystallinity and crystal size decreased as CMC addition increased. FESEM results showed CMC-altered BC produced from agitated culture retained its interweaving property. TGA results demonstrated that CMC-altered BC had about 98% water retention ability, which is higher than BC pellicle produced with static culture. CMC-altered BC also exhibited higher Tmax compared to control. Finally, DMA results showed that BC from agitated culture loses its mechanical strength in both stress at break and Young's modulus when compared to BC pellicle. This study clearly demonstrated that addition of CMC enhanced BC production and slightly changed its structure.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Pages2003-2013
Number of pages11
StatePublished - Dec 1 2009
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009 - Reno, NV, United States
Duration: Jun 21 2009Jun 24 2009

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009
Volume3

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2009
CountryUnited States
CityReno, NV
Period6/21/096/24/09

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All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Cheng, K. C., Catchmark, J., & Demirci, A. (2009). Enhanced production of bacterial cellulose under agitated condition and its material property analysis. In American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009 (pp. 2003-2013). (American Society of Agricultural and Biological Engineers Annual International Meeting 2009, ASABE 2009; Vol. 3).