Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

H. Steven Seifert, Ronald D. Porter

Research output: Contribution to journalArticle

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F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalMGG Molecular & General Genetics
Issue number2
Publication statusPublished - Feb 1 1984


All Science Journal Classification (ASJC) codes

  • Genetics

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