Enhanced recombination between λp lac5 and mini-F-lac: The tra regulon is required for recombination enhancement

H. Steven Seifert, Ronald D. Porter

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalMGG Molecular & General Genetics
Volume193
Issue number2
DOIs
StatePublished - Feb 1 1984

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Regulon
Genetic Recombination
Lac Operon
F Factor
Plasmids

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.",
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Enhanced recombination between λp lac5 and mini-F-lac : The tra regulon is required for recombination enhancement. / Seifert, H. Steven; Porter, Ronald D.

In: MGG Molecular & General Genetics, Vol. 193, No. 2, 01.02.1984, p. 269-274.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhanced recombination between λp lac5 and mini-F-lac

T2 - The tra regulon is required for recombination enhancement

AU - Seifert, H. Steven

AU - Porter, Ronald D.

PY - 1984/2/1

Y1 - 1984/2/1

N2 - F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

AB - F42 lac recombination with λp lac is normally 20-fold to 50-fold higher than recombination between λp lac and a chromosomal lac gene. Transductional crosses with λp lac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42 lac.

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