Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins

Emily E. Helliwell, Julio Vega-Arreguín, Zi Shi, Bryan Bailey, Shunyuan Xiao, Siela N. Maximova, Brett M. Tyler, Mark J. Guiltinan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T. cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T. cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P. tropicalis and P. palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T. cacao, and potentially in other plants, against a broad spectrum of pathogens.

Original languageEnglish (US)
Pages (from-to)875-886
Number of pages12
JournalPlant Biotechnology Journal
Volume14
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Phosphate-Binding Proteins
Oomycetes
Theobroma cacao
Cacao
phosphatidylinositols
binding proteins
secretion
phosphates
pathogens
Phytophthora palmivora
Phytophthora
Colletotrichum
lesions (plant)
Proteins
Disease Resistance
proteins
Plant Cells
chocolate
disease resistance
phosphatidylinositol 3-phosphate

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Helliwell, Emily E. ; Vega-Arreguín, Julio ; Shi, Zi ; Bailey, Bryan ; Xiao, Shunyuan ; Maximova, Siela N. ; Tyler, Brett M. ; Guiltinan, Mark J. / Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins. In: Plant Biotechnology Journal. 2016 ; Vol. 14, No. 3. pp. 875-886.
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Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins. / Helliwell, Emily E.; Vega-Arreguín, Julio; Shi, Zi; Bailey, Bryan; Xiao, Shunyuan; Maximova, Siela N.; Tyler, Brett M.; Guiltinan, Mark J.

In: Plant Biotechnology Journal, Vol. 14, No. 3, 01.03.2016, p. 875-886.

Research output: Contribution to journalArticle

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