Enhanced thermal destruction of Listeria monocytogenes and Staphylococcus aureus by the lactoperoxidase system

D. N. Kamau, Stephanie Doores, K. M. Pruitt

Research output: Contribution to journalArticle

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Abstract

The lactoperoxidase system (LPS) enhanced thermal destruction of Listeria monocytogenes and Staphylococcus aureus. After LPS activation, biphasic survival curves were observed for L. monocytogenes at 57.8°C and for S. aureus at 55.2°C. The data were consistent with a model that assumed two bacterial populations differing in heat sensitivity. The more heat-sensitive fractions (93% of the L. monocytogenes, 92% of the S. aureus) were killed almost instantly. For these biphasic survival curves, D values were based on the much smaller, less-heat-sensitive fractions. For L. ytogenes, the D(52.2°C) values were 30.2 min (untreated milk) and 10.7 min (LPS activated); corresponding D(55.2°C) values were 8.2 and 1.6 min; corresponding D(57.8°C) values were 2.3 and 0.5 min. For S aureus, the D(52.2°C) values were 33.3 min (untreated milk) and 2.2 min (LPS activated), and the corresponding D(55.2°C) values were 7.6 and 1.1 min, respectively. The most rapid killing of L. monocytogenes occurred when samples were heated soon after activation of the LPS. Activation of the LPS followed by heating can increase the margin of safety with respect to milkborne pathogens.

Original languageEnglish (US)
Pages (from-to)2711-2716
Number of pages6
JournalApplied and environmental microbiology
Volume56
Issue number9
StatePublished - Sep 20 1990

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Incineration
Lactoperoxidase
Listeria monocytogenes
milk
Staphylococcus aureus
peroxidase
heat
pathogen
Hot Temperature
safety
heating
Milk
margin of safety
Heating
Safety
pathogens
Population

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

@article{86015238120342b28d51ebcb929b70d1,
title = "Enhanced thermal destruction of Listeria monocytogenes and Staphylococcus aureus by the lactoperoxidase system",
abstract = "The lactoperoxidase system (LPS) enhanced thermal destruction of Listeria monocytogenes and Staphylococcus aureus. After LPS activation, biphasic survival curves were observed for L. monocytogenes at 57.8°C and for S. aureus at 55.2°C. The data were consistent with a model that assumed two bacterial populations differing in heat sensitivity. The more heat-sensitive fractions (93{\%} of the L. monocytogenes, 92{\%} of the S. aureus) were killed almost instantly. For these biphasic survival curves, D values were based on the much smaller, less-heat-sensitive fractions. For L. ytogenes, the D(52.2°C) values were 30.2 min (untreated milk) and 10.7 min (LPS activated); corresponding D(55.2°C) values were 8.2 and 1.6 min; corresponding D(57.8°C) values were 2.3 and 0.5 min. For S aureus, the D(52.2°C) values were 33.3 min (untreated milk) and 2.2 min (LPS activated), and the corresponding D(55.2°C) values were 7.6 and 1.1 min, respectively. The most rapid killing of L. monocytogenes occurred when samples were heated soon after activation of the LPS. Activation of the LPS followed by heating can increase the margin of safety with respect to milkborne pathogens.",
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Enhanced thermal destruction of Listeria monocytogenes and Staphylococcus aureus by the lactoperoxidase system. / Kamau, D. N.; Doores, Stephanie; Pruitt, K. M.

In: Applied and environmental microbiology, Vol. 56, No. 9, 20.09.1990, p. 2711-2716.

Research output: Contribution to journalArticle

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