Enhancing the specificity of peptide-nucleic acid binding with DNA

Maria Krasilnikova, A. G. Veselkov

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The specificity of peptide-nucleic acid (PNA) binding with DNA could be enhanced at the expense of lower thermal stability of nonspecific PNA-DNA complexes. Using a model complex of TTCTTCTTTT PNA and phage λ DNA, the bulk of nonspecific complexes were shown to dissociate at lower temperatures. However, some nonspecific complexes had almost the stability as the specific ones. The DNA sites admitting such stable nonspecific complexes were located; sequence analysis suggested that these were DNA stretches with terminal mismatches. The technique for locating PNA-DNA binding sites was improved to permit handling of long DNA molecules (up to 50 kbp).

Original languageEnglish (US)
Pages (from-to)226-230
Number of pages5
JournalMolecular Biology
Volume30
Issue number29992
StatePublished - Mar 1996

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics

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