Enhancing the stability of kinesin motors for microscale transport applications

Maruti Uppalapati, Ying Ming Huang, Thomas Nelson Jackson, William Olaf Hancock

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Biomolecular motors, such as kinesins, have great potential for micro-actuation and micro- or nanoscale active transport when integrated into microscale devices. However, the stability and limited shelf life of these motor proteins and their associated protein filaments is a barrier to their implementation. Here we demonstrate that freeze-drying or critical point-drying kinesins adsorbed to glass surfaces extends their lifetime from days to more than four months. Further, photoresist deposition and removal can be carried out on these motor-adsorbed surfaces without loss of motor function. The methods developed here are an important step towards realizing the integration of biological motors into practical devices, and these approaches can be extended to patterning and preserving other proteins immobilized on surfaces.

Original languageEnglish (US)
Pages (from-to)358-361
Number of pages4
JournalLab on a Chip
Volume8
Issue number2
DOIs
StatePublished - Jan 1 2008

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this