Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice

Yuan Wan, Mackenzie Maurer, Hong Zhang He, Yi Qiu Xia, Wen Long Zhang, Si Jie Hao, Nelson Shu-Sang Yee, Siyang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.

Original languageEnglish (US)
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages273-276
Number of pages4
ISBN (Electronic)9781728120072
DOIs
StatePublished - Jun 1 2019
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: Jun 23 2019Jun 27 2019

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period6/23/196/27/19

Fingerprint

Nanoprobes
Vesicles
Lipids
Silica
Silicon Dioxide
lipids
Blood Proteins
Nanostructures
isolation
Impurities
silicon dioxide
Plasmas
Isolation
Monitoring
tethering
Proteins
Plasma
contaminants
platforms
cancer

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

Cite this

Wan, Y., Maurer, M., He, H. Z., Xia, Y. Q., Zhang, W. L., Hao, S. J., ... Zheng, S. (2019). Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 273-276). [8808761] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808761
Wan, Yuan ; Maurer, Mackenzie ; He, Hong Zhang ; Xia, Yi Qiu ; Zhang, Wen Long ; Hao, Si Jie ; Yee, Nelson Shu-Sang ; Zheng, Siyang. / Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 273-276 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
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abstract = "Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97{\%}.",
author = "Yuan Wan and Mackenzie Maurer and He, {Hong Zhang} and Xia, {Yi Qiu} and Zhang, {Wen Long} and Hao, {Si Jie} and Yee, {Nelson Shu-Sang} and Siyang Zheng",
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Wan, Y, Maurer, M, He, HZ, Xia, YQ, Zhang, WL, Hao, SJ, Yee, NS-S & Zheng, S 2019, Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808761, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 273-276, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 6/23/19. https://doi.org/10.1109/TRANSDUCERS.2019.8808761

Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. / Wan, Yuan; Maurer, Mackenzie; He, Hong Zhang; Xia, Yi Qiu; Zhang, Wen Long; Hao, Si Jie; Yee, Nelson Shu-Sang; Zheng, Siyang.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 273-276 8808761 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Wan, Yuan

AU - Maurer, Mackenzie

AU - He, Hong Zhang

AU - Xia, Yi Qiu

AU - Zhang, Wen Long

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AU - Yee, Nelson Shu-Sang

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AB - Extracellular vesicles (EVs), from clinical plasma samples, have recently been used towards applications of cancer diagnostics and treatment monitoring. However, clinical translation has been limited by technical challenges in EV enrichment. Thus, we have reported on the development of a microdevice platform that utilizes dual capture principles for improved EV isolation and enrichment. By utilizing (1) an EV-specific lipid nanoprobe-grafted silica for isolation via EV tethering and (2) EV size-matched nanostructures for isolation via physical trapping, isolation efficiency is increased by nearly three times. Furthermore, this continuous-flow microdevice eliminates plasma protein contaminants by upwards of ~97%.

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ER -

Wan Y, Maurer M, He HZ, Xia YQ, Zhang WL, Hao SJ et al. Enrichment of Extracellular Vesicles Via Lipid Nanoprobe-Functionalized Nanostructured Silica Microdevice. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 273-276. 8808761. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808761