Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

Yuan Wan; MacKenzie Maurer; Hong Zhang He; Yi Qiu Xia; Si Jie Hao; Wen Long Zhang; Nelson S. Yee; Si Yang Zheng

doi:10.1039/c8lc01359d

Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

Yuan Wan, MacKenzie Maurer, Hong Zhang He, Yi Qiu Xia, Si Jie Hao, Wen Long Zhang, Nelson S. Yee, Si Yang Zheng

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.

Original language	English (US)
Pages (from-to)	2346-2355
Number of pages	10
Journal	Lab on a Chip
Volume	19
Issue number	14
DOIs	https://doi.org/10.1039/c8lc01359d
State	Published - Jul 21 2019

All Science Journal Classification (ASJC) codes

Bioengineering
Biochemistry
Chemistry(all)
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1039/c8lc01359d

Cite this

@article{9eba36e9fa0846bfaa98a698906ae5d1,

title = "Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica",

abstract = "Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.",

author = "Yuan Wan and MacKenzie Maurer and He, {Hong Zhang} and Xia, {Yi Qiu} and Hao, {Si Jie} and Zhang, {Wen Long} and Yee, {Nelson S.} and Zheng, {Si Yang}",

note = "Funding Information: This work was supported by the National Cancer Institute of the National Institutes of Health under grant number 1R01CA230339 awarded to S. Y. Zheng. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

month = jul,

day = "21",

doi = "10.1039/c8lc01359d",

language = "English (US)",

volume = "19",

pages = "2346--2355",

journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",

issn = "1473-0197",

publisher = "Royal Society of Chemistry",

number = "14",

}

TY - JOUR

T1 - Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

AU - Wan, Yuan

AU - Maurer, MacKenzie

AU - He, Hong Zhang

AU - Xia, Yi Qiu

AU - Hao, Si Jie

AU - Zhang, Wen Long

AU - Yee, Nelson S.

AU - Zheng, Si Yang

N1 - Funding Information: This work was supported by the National Cancer Institute of the National Institutes of Health under grant number 1R01CA230339 awarded to S. Y. Zheng. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019/7/21

Y1 - 2019/7/21

N2 - Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.

AB - Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.

UR - http://www.scopus.com/inward/record.url?scp=85068797544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068797544&partnerID=8YFLogxK

U2 - 10.1039/c8lc01359d

DO - 10.1039/c8lc01359d

M3 - Article

C2 - 31232418

AN - SCOPUS:85068797544

SN - 1473-0197

VL - 19

SP - 2346

EP - 2355

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

IS - 14

ER -

Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this