Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

Yuan Wan, MacKenzie Maurer, Hong Zhang He, Yi Qiu Xia, Si Jie Hao, Wen Long Zhang, Nelson S. Yee, Si Yang Zheng

Research output: Contribution to journalArticle

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Abstract

Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS, in nEV DNA from the plasma of pancreatic cancer patients.

Original languageEnglish (US)
Pages (from-to)2346-2355
Number of pages10
JournalLab on a Chip
Volume19
Issue number14
DOIs
StatePublished - Jan 1 2019

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Wan, Y., Maurer, M., He, H. Z., Xia, Y. Q., Hao, S. J., Zhang, W. L., Yee, N. S., & Zheng, S. Y. (2019). Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica. Lab on a Chip, 19(14), 2346-2355. https://doi.org/10.1039/c8lc01359d