Enterovirus 71 Binding to PSGL-1 on Leukocytes: VP1-145 Acts as a Molecular Switch to Control Receptor Interaction

Yorihiro Nishimura, Hyunwook Lee, Susan Hafenstein, Chikako Kataoka, Takaji Wakita, Jeffrey M. Bergelson, Hiroyuki Shimizu

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Some strains of enterovirus 71 (EV71), but not others, infect leukocytes by binding to a specific receptor molecule: the P-selectin glycoprotein ligand-1 (PSGL-1). We find that a single amino acid residue within the capsid protein VP1 determines whether EV71 binds to PSGL-1. Examination of capsid sequences of representative EV71 strains revealed that the PSGL-1-binding viruses had either a G or a Q at residue 145 within the capsid protein VP1 (VP1-145G or Q), whereas PSGL-1-nonbinding viruses had VP1-145E. Using site-directed mutagenesis we found that PSGL-1-binding strains lost their capacity to bind when VP1-145G/Q was replaced by E; conversely, nonbinding strains gained the capacity to bind PSGL-1 when VP1-145E was replaced with either G or Q. Viruses with G/Q at VP1-145 productively infected a leukocyte cell line, Jurkat T-cells, whereas viruses with E at this position did not. We previously reported that EV71 binds to the N-terminal region of PSGL-1, and that binding depends on sulfated tyrosine residues within this region. We speculated that binding depends on interaction between negatively charged sulfate groups and positively charged basic residues in the virus capsid. VP1-145 on the virus surface is in close proximity to conserved lysine residues at VP1-242 and VP1-244. Comparison of recently published crystal structures of EV71 isolates with either Q or E at VP1-145 revealed that VP1-145 controls the orientation of the lysine side-chain of VP1-244: with VP1-145Q the lysine side chain faces outward, but with VP1-145E, the lysine side chain is turned toward the virus surface. Mutation of VP1-244 abolished virus binding to PSGL-1, and mutation of VP1-242 greatly reduced binding. We propose that conserved lysine residues on the virus surface are responsible for interaction with sulfated tyrosine residues at the PSGL-1 N-terminus, and that VP1-145 acts as a switch, controlling PSGL-1 binding by modulating the exposure of VP1-244K.

Original languageEnglish (US)
Article numbere1003511
JournalPLoS pathogens
Volume9
Issue number7
DOIs
StatePublished - Jul 1 2013

Fingerprint

Enterovirus
Leukocytes
Viruses
Lysine
Virus Attachment
Capsid
Capsid Proteins
Tyrosine
P-selectin ligand protein
Mutation
Jurkat Cells
Site-Directed Mutagenesis
Sulfates
T-Lymphocytes
Amino Acids
Cell Line

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Nishimura, Yorihiro ; Lee, Hyunwook ; Hafenstein, Susan ; Kataoka, Chikako ; Wakita, Takaji ; Bergelson, Jeffrey M. ; Shimizu, Hiroyuki. / Enterovirus 71 Binding to PSGL-1 on Leukocytes : VP1-145 Acts as a Molecular Switch to Control Receptor Interaction. In: PLoS pathogens. 2013 ; Vol. 9, No. 7.
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Enterovirus 71 Binding to PSGL-1 on Leukocytes : VP1-145 Acts as a Molecular Switch to Control Receptor Interaction. / Nishimura, Yorihiro; Lee, Hyunwook; Hafenstein, Susan; Kataoka, Chikako; Wakita, Takaji; Bergelson, Jeffrey M.; Shimizu, Hiroyuki.

In: PLoS pathogens, Vol. 9, No. 7, e1003511, 01.07.2013.

Research output: Contribution to journalArticle

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AU - Nishimura, Yorihiro

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AU - Hafenstein, Susan

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