Enumeration of chlorine-stressed organisms with acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (AOINT)

Diane S. Herson, Balkumar Marthi, Mary Anne Payer, Katherine H. Baker

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Direct microscopic enumeration of Enterobacter cloacae with the acridine orange 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride technique (AOINT) was compared with spread plate counts on nonselective media to establish the usefulness of the former technique in the enumeration of chlorine-stressed cells. Results indicate that the techniques are comparable when the organisms are not stressed. However, AOINT is more sensitive than are plate counts in the detection of chlorine-stressed cells.

Original languageEnglish (US)
Pages (from-to)77-80
Number of pages4
JournalCurrent Microbiology
Volume13
Issue number2
DOIs
StatePublished - Mar 1 1986

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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