Environment determines fidelity for an RNA virus replicase

Justin S. Pita, Joachim R. De Miranda, William L. Schneider, Marilyn J. Roossinck

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The rate of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined in planta by using a parasitic satellite RNA (satRNA) as a reporter. We found that the CMV replicase had different fidelity in different environments, with important implications in viral disease evolution. Insertions were very rare events, irrespective of the region of the satRNA genome assayed and independent of the hosts tested. On the other hand, deletion events were more frequent but were restricted to a highly structured region of the reporter. Deletion mutation rates were different for the two hosts tested, although the mutation distribution was not influenced by the hosts. Moreover, hot spots with high mutation rates were identified on the satRNA genome.

Original languageEnglish (US)
Pages (from-to)9072-9077
Number of pages6
JournalJournal of virology
Volume81
Issue number17
DOIs
StatePublished - Sep 1 2007

Fingerprint

Satellite RNAs
satellite RNA
RNA Replicase
RNA Viruses
Cucumovirus
Cucumber mosaic virus
Mutation Rate
INDEL Mutation
Genome
mutation
genome
Sequence Deletion
Virus Diseases
Plantae
Mutation
RNA viruses
sequence deletion

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Pita, Justin S. ; De Miranda, Joachim R. ; Schneider, William L. ; Roossinck, Marilyn J. / Environment determines fidelity for an RNA virus replicase. In: Journal of virology. 2007 ; Vol. 81, No. 17. pp. 9072-9077.
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Environment determines fidelity for an RNA virus replicase. / Pita, Justin S.; De Miranda, Joachim R.; Schneider, William L.; Roossinck, Marilyn J.

In: Journal of virology, Vol. 81, No. 17, 01.09.2007, p. 9072-9077.

Research output: Contribution to journalArticle

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