Environment determines fidelity for an RNA virus replicase

Justin S. Pita, Joachim R. De Miranda, William L. Schneider, Marilyn J. Roossinck

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The rate of insertion and deletion mutations of the replicase of Cucumber mosaic virus (CMV) was determined in planta by using a parasitic satellite RNA (satRNA) as a reporter. We found that the CMV replicase had different fidelity in different environments, with important implications in viral disease evolution. Insertions were very rare events, irrespective of the region of the satRNA genome assayed and independent of the hosts tested. On the other hand, deletion events were more frequent but were restricted to a highly structured region of the reporter. Deletion mutation rates were different for the two hosts tested, although the mutation distribution was not influenced by the hosts. Moreover, hot spots with high mutation rates were identified on the satRNA genome.

Original languageEnglish (US)
Pages (from-to)9072-9077
Number of pages6
JournalJournal of virology
Volume81
Issue number17
DOIs
StatePublished - Sep 2007

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint Dive into the research topics of 'Environment determines fidelity for an RNA virus replicase'. Together they form a unique fingerprint.

Cite this