Environmental differences in hemoglobin gene expression in the hydrothermal vent tubeworm, Ridgeia piscesae

Susan L. Carney, Jason F. Flores, Kathryn M. Orobona, David A. Butterfield, Charles R. Fisher, Stephen W. Schaeffer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Ridgeia piscesae, the siboglinid tubeworm inhabiting the hydrothermal vents of the northeast Pacific Juan de Fuca Ridge, displays a wide range of microhabitat-specific, genetically indistinguishable phenotypes. Local microhabitat conditions are hypothesized to play a role in the differentiation of R. piscesae phenotypes. Extracellular hemoglobins serve to connect the tubeworm and the surrounding vent fluid, binding environmental sulfide and oxygen for transport to endosymbionts that use the chemical energy for carbon fixation. Because hemoglobin is essential for this symbiosis, we examined its expression in two of the most extreme R. piscesae phenotypes at two levels: the mRNA encoding the globin subunits and the whole molecules in coelomic and vascular fluids. Levels of gene expression were up to 12 times greater in short-fat R. piscesae from higher temperature, sulfide chimney environments compared to long-skinny animals from a low temperature, diffuse flow basalt habitat. Gene expression levels were consistent with the relative concentrations of hemoglobin molecules in the vascular and coelomic fluids. Up to a 20-fold variation in globin gene expression was detected between the same phenotype from different sites. These data demonstrate that local environmental factors influence not only phenotype but gene expression and its resulting physiological outcome within this unique species.

Original languageEnglish (US)
Pages (from-to)326-337
Number of pages12
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume146
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Hydrothermal Vents
Vents
Gene expression
Hemoglobins
Phenotype
Gene Expression
Globins
Sulfides
Fluids
Blood Vessels
Chimneys
Molecules
Carbon Cycle
Temperature
Symbiosis
Animals
Carbon
Fats
Ecosystem
Oxygen

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

Carney, Susan L. ; Flores, Jason F. ; Orobona, Kathryn M. ; Butterfield, David A. ; Fisher, Charles R. ; Schaeffer, Stephen W. / Environmental differences in hemoglobin gene expression in the hydrothermal vent tubeworm, Ridgeia piscesae. In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 2007 ; Vol. 146, No. 3. pp. 326-337.
@article{96ab691a092b42d88e87ce3723310118,
title = "Environmental differences in hemoglobin gene expression in the hydrothermal vent tubeworm, Ridgeia piscesae",
abstract = "Ridgeia piscesae, the siboglinid tubeworm inhabiting the hydrothermal vents of the northeast Pacific Juan de Fuca Ridge, displays a wide range of microhabitat-specific, genetically indistinguishable phenotypes. Local microhabitat conditions are hypothesized to play a role in the differentiation of R. piscesae phenotypes. Extracellular hemoglobins serve to connect the tubeworm and the surrounding vent fluid, binding environmental sulfide and oxygen for transport to endosymbionts that use the chemical energy for carbon fixation. Because hemoglobin is essential for this symbiosis, we examined its expression in two of the most extreme R. piscesae phenotypes at two levels: the mRNA encoding the globin subunits and the whole molecules in coelomic and vascular fluids. Levels of gene expression were up to 12 times greater in short-fat R. piscesae from higher temperature, sulfide chimney environments compared to long-skinny animals from a low temperature, diffuse flow basalt habitat. Gene expression levels were consistent with the relative concentrations of hemoglobin molecules in the vascular and coelomic fluids. Up to a 20-fold variation in globin gene expression was detected between the same phenotype from different sites. These data demonstrate that local environmental factors influence not only phenotype but gene expression and its resulting physiological outcome within this unique species.",
author = "Carney, {Susan L.} and Flores, {Jason F.} and Orobona, {Kathryn M.} and Butterfield, {David A.} and Fisher, {Charles R.} and Schaeffer, {Stephen W.}",
year = "2007",
month = "3",
day = "1",
doi = "10.1016/j.cbpb.2006.11.002",
language = "English (US)",
volume = "146",
pages = "326--337",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "3",

}

Environmental differences in hemoglobin gene expression in the hydrothermal vent tubeworm, Ridgeia piscesae. / Carney, Susan L.; Flores, Jason F.; Orobona, Kathryn M.; Butterfield, David A.; Fisher, Charles R.; Schaeffer, Stephen W.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 146, No. 3, 01.03.2007, p. 326-337.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Environmental differences in hemoglobin gene expression in the hydrothermal vent tubeworm, Ridgeia piscesae

AU - Carney, Susan L.

AU - Flores, Jason F.

AU - Orobona, Kathryn M.

AU - Butterfield, David A.

AU - Fisher, Charles R.

AU - Schaeffer, Stephen W.

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Ridgeia piscesae, the siboglinid tubeworm inhabiting the hydrothermal vents of the northeast Pacific Juan de Fuca Ridge, displays a wide range of microhabitat-specific, genetically indistinguishable phenotypes. Local microhabitat conditions are hypothesized to play a role in the differentiation of R. piscesae phenotypes. Extracellular hemoglobins serve to connect the tubeworm and the surrounding vent fluid, binding environmental sulfide and oxygen for transport to endosymbionts that use the chemical energy for carbon fixation. Because hemoglobin is essential for this symbiosis, we examined its expression in two of the most extreme R. piscesae phenotypes at two levels: the mRNA encoding the globin subunits and the whole molecules in coelomic and vascular fluids. Levels of gene expression were up to 12 times greater in short-fat R. piscesae from higher temperature, sulfide chimney environments compared to long-skinny animals from a low temperature, diffuse flow basalt habitat. Gene expression levels were consistent with the relative concentrations of hemoglobin molecules in the vascular and coelomic fluids. Up to a 20-fold variation in globin gene expression was detected between the same phenotype from different sites. These data demonstrate that local environmental factors influence not only phenotype but gene expression and its resulting physiological outcome within this unique species.

AB - Ridgeia piscesae, the siboglinid tubeworm inhabiting the hydrothermal vents of the northeast Pacific Juan de Fuca Ridge, displays a wide range of microhabitat-specific, genetically indistinguishable phenotypes. Local microhabitat conditions are hypothesized to play a role in the differentiation of R. piscesae phenotypes. Extracellular hemoglobins serve to connect the tubeworm and the surrounding vent fluid, binding environmental sulfide and oxygen for transport to endosymbionts that use the chemical energy for carbon fixation. Because hemoglobin is essential for this symbiosis, we examined its expression in two of the most extreme R. piscesae phenotypes at two levels: the mRNA encoding the globin subunits and the whole molecules in coelomic and vascular fluids. Levels of gene expression were up to 12 times greater in short-fat R. piscesae from higher temperature, sulfide chimney environments compared to long-skinny animals from a low temperature, diffuse flow basalt habitat. Gene expression levels were consistent with the relative concentrations of hemoglobin molecules in the vascular and coelomic fluids. Up to a 20-fold variation in globin gene expression was detected between the same phenotype from different sites. These data demonstrate that local environmental factors influence not only phenotype but gene expression and its resulting physiological outcome within this unique species.

UR - http://www.scopus.com/inward/record.url?scp=33847227354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847227354&partnerID=8YFLogxK

U2 - 10.1016/j.cbpb.2006.11.002

DO - 10.1016/j.cbpb.2006.11.002

M3 - Article

C2 - 17240180

AN - SCOPUS:33847227354

VL - 146

SP - 326

EP - 337

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 3

ER -