TY - JOUR
T1 - EnvZ/OmpR two-component signaling
T2 - An archetype system that can function noncanonically
AU - Kenney, Linda J.
AU - Anand, Ganesh S.
N1 - Funding Information:
We thank Melanie Lee and Diego Pitta de Araujo, MBI SciComm, Jeremy, Loo Chien Wang, Nikhil Kumar Tulsian, Department of Biological Sciences, NUS, for Figs. 2, 8, and Dasvit Shetty for Fig. 5A. This work was supported by NIH grants AI123645, VAIOBX-000372, and MOE2018-T2-1-038 to L.J.K. and a Research Centre of Excellence grant in mechanobiology.
Funding Information:
This work was supported by NIH grants AI123645, VAIOBX-000372, and MOE2018-T2-1-038 to L.J.K. and a Research Centre of Excellence grant in mechanobiology.
Publisher Copyright:
© 2020 American Society for Microbiology.
PY - 2020
Y1 - 2020
N2 - Two-component regulatory systems represent the major paradigm for signal transduction in prokaryotes. The simplest systems are composed of a sensor kinase and a response regulator. The sensor is often a membrane protein that senses a change in environmental conditions and is autophosphorylated by ATP on a histidine residue. The phosphoryl group is transferred onto an aspartate of the response regulator, which activates the regulator and alters its output, usually resulting in a change in gene expression. In this review, we present a historical view of the archetype EnvZ/OmpR two-component signaling system, and then we provide a new view of signaling based on our recent experiments. EnvZ responds to cytoplasmic signals that arise from changes in the extracellular milieu, and OmpR acts canonically (requiring phosphorylation) to regulate the porin genes and noncanonically (without phosphorylation) to activate the acid stress response. Herein, we describe how insights gleaned from stimulus recognition and response in EnvZ are relevant to nearly all sensor kinases and response regulators.
AB - Two-component regulatory systems represent the major paradigm for signal transduction in prokaryotes. The simplest systems are composed of a sensor kinase and a response regulator. The sensor is often a membrane protein that senses a change in environmental conditions and is autophosphorylated by ATP on a histidine residue. The phosphoryl group is transferred onto an aspartate of the response regulator, which activates the regulator and alters its output, usually resulting in a change in gene expression. In this review, we present a historical view of the archetype EnvZ/OmpR two-component signaling system, and then we provide a new view of signaling based on our recent experiments. EnvZ responds to cytoplasmic signals that arise from changes in the extracellular milieu, and OmpR acts canonically (requiring phosphorylation) to regulate the porin genes and noncanonically (without phosphorylation) to activate the acid stress response. Herein, we describe how insights gleaned from stimulus recognition and response in EnvZ are relevant to nearly all sensor kinases and response regulators.
UR - http://www.scopus.com/inward/record.url?scp=85078710727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85078710727&partnerID=8YFLogxK
U2 - 10.1128/ecosalplus.ESP-0001-2019
DO - 10.1128/ecosalplus.ESP-0001-2019
M3 - Article
C2 - 32003321
AN - SCOPUS:85078710727
VL - 9
SP - 1
EP - 30
JO - EcoSal Plus
JF - EcoSal Plus
SN - 2324-6200
IS - 1
ER -